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Rat Extracellular Matrix Metalloproteinase Inducer (EMMPRIN) ELISA Kit

SKU:
RTDL00329
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P26453
Sensitivity:
5.5pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
CD147,BSG,5F7,M6,OK,TCSF,Basigin(Ok Blood Group),Collagenase stimulatory factor,OK blood group antigen,Tumor cell-derived collagenase stimulatory factor
Reactivity:
Rat
Sample Type:
Serum, Plasma, Lung Lavage Fluid And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Rat Extracellular Matrix Metalloproteinase Inducer (EMMPRIN) ELISA Kit

The Rat Extracellular Matrix Metalloproteinase Inducer (EMMPRIN) ELISA Kit is specifically designed for the accurate detection of EMMPRIN levels in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.EMMPRIN, also known as CD147, is a protein that plays a critical role in facilitating tumor cell invasion and metastasis by inducing the expression of matrix metalloproteinases (MMPs).

It is also involved in various physiological and pathological processes, including inflammation, tissue remodeling, and angiogenesis.This ELISA kit is a valuable tool for studying the role of EMMPRIN in cancer progression, cardiovascular diseases, and other related conditions, as well as for developing potential therapeutic interventions. Its ease of use and accurate results make it an essential component for researchers investigating EMMPRIN biology and its implications in disease.

Product Name:Rat Extracellular Matrix Metalloproteinase Inducer (EMMPRIN) ELISA Kit
Product Code:RTDL00329
Size:96 Assays
Target:EMMPRIN
Synonyms:CD147, BSG, 5F7, M6, OK, TCSF, Basigin(Ok Blood Group), Collagenase stimulatory factor, OK blood group antigen, Tumor cell-derived collagenase stimulatory factor
Detection Method:Sandwich
Range:15.6-1,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10191
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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