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Rat Estrogen Receptor Alpha (ERa) ELISA Kit

SKU:
RTDL00336
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P06211
Sensitivity:
0.117ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
ER-A1,ER,ESR,ESR1,ESRA,NR3A1,NR3-A1,Estrogen Receptor 1,Nuclear Receptor Subfamily 3,Group A,Member 1
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Rat Estrogen Receptor Alpha (ERa) ELISA Kit

The Rat Estrogen Receptor Alpha (ERα) ELISA Kit is specifically designed for the precise measurement of ERα levels in rat samples, including serum, plasma, and tissue lysates. This kit offers superior sensitivity and specificity, ensuring accurate and consistent results for various research applications.ERα is a key player in the regulation of estrogen signaling, influencing gene expression and cellular functions. Dysregulation of ERα has been implicated in various diseases, including breast cancer, osteoporosis, and cardiovascular disorders, underscoring its importance as a therapeutic target and biomarker for disease progression.

With its reliable performance and ease of use, the Rat ERα ELISA Kit is an essential tool for studying estrogen receptor signaling pathways and developing targeted therapies for ERα-related diseases. Trust in this kit to deliver reliable results for your research needs.

Product Name:Rat Estrogen Receptor Alpha (ERa) ELISA Kit
Product Code:RTDL00336
Size:96 Assays
Target:ERa
Synonyms:ER-A1, ER, ESR, ESR1, ESRA, NR3A1, NR3-A1, Estrogen Receptor 1, Nuclear Receptor Subfamily 3,Group A,Member 1
Detection Method:Sandwich
Range:0.625-40ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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