The Rat EPO (Erythropoietin) ELISA Kit is a specialized assay designed for the quantitative detection of Erythropoietin levels in rat biological samples. Erythropoietin, a critical hormone produced by the kidneys, plays a pivotal role in red blood cell production and regulation.
This ELISA kit provides exceptional sensitivity and specificity, enabling accurate measurement of Erythropoietin concentrations in rat samples. Erythropoietin is essential for maintaining normal red blood cell levels and is involved in responding to hypoxia and regulating erythropoiesis. Manufactured under stringent quality control standards, this ELISA kit offers robust performance, reliable results, and ease of use, making it an excellent choice for researchers studying erythropoiesis, hematopoiesis, and other biological processes influenced by EPO levels.
Product Name:
Rat EPO (Erythropoietin) ELISA Kit
SKU:
AEES00290
Target:
Rat EPO (Erythropoietin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat EPO. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat EPO and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat EPO, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat EPO. You can calculate the concentration of Rat EPO in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-101
91-103
92-107
Average (%)
92
96
99
1:4
Range (%)
98-112
84-94
98-110
Average (%)
103
89
103
1:8
Range (%)
101-114
86-100
93-106
Average (%)
108
91
99
1:16
Range (%)
100-114
87-102
92-106
Average (%)
108
94
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-101
95
EDTA plasma (n=5)
95-111
101
Cell culture media (n=5)
88-103
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
221.7
514.3
1746.7
219.9
557.7
1706.1
Standard deviation
11.3
30.9
76.9
14.7
25.7
83.6
C V (%)
5.1
6.01
4.4
6.68
4.61
4.9
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat EPO concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat EPO in samples. No significant cross-reactivity or interference between Rat EPO and analogues was observed.
