The Rat EPO (Erythropoietin) CLIA Kit is specifically designed for the precise measurement of erythropoietin levels in rat serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit guarantees accurate and consistent results, making it a valuable tool for various research purposes.Erythropoietin is a vital hormone that stimulates red blood cell production and is crucial for oxygen transport in the body.
Aberrant levels of erythropoietin have been linked to various conditions such as anemia, kidney disease, and cancer, highlighting its significance as a biomarker for disease diagnosis and treatment assessment. By utilizing the Rat EPO CLIA Kit, researchers can effectively study erythropoietin biology and its implications in disease pathogenesis, ultimately aiding in the development of innovative therapeutic strategies.
Product Name:
Rat EPO (Erythropoietin) CLIA Kit
SKU:
RTES00007
Target:
Rat EPO (Erythropoietin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat EPO. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat EPO and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat EPO, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat EPO. You can calculate the concentration of Rat EPO in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-113
94-111
101-116
Average (%)
106
101
107
1:4
Range (%)
102-117
83-97
91-107
Average (%)
110
90
98
1:8
Range (%)
87-98
91-103
91-104
Average (%)
92
97
96
1:16
Range (%)
90-106
101-119
88-101
Average (%)
98
109
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-111
104
EDTA plasma (n=5)
96-109
101
Cell culture media (n=5)
84-98
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
45.51
145.97
398.85
49.45
134.87
437.14
Standard deviation
5.65
14.61
31.31
6.27
10.44
33.97
C V (%)
12.41
10.01
7.85
12.68
7.74
7.77
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat EPO concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat EPO in samples. No significant cross-reactivity or interference between Rat EPO and analogues was observed.
