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Rat β-EP (Beta-Endorphin) ELISA Kit (AEES00324)

SKU:
AEES00324
Product Type:
ELISA Kit
ELISA Type:
Competitive
Size:
96 Assays
Reactivity:
Rat
Sensitivity:
9.38 pg/mL
Range:
15.63-1000 pg/mL
Sample Type:
Serum, plasma and other biological fluids
$599
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Rat β-EP (Beta-Endorphin) ELISA Kit
SKU:AEES00324
Target:Rat β-EP (Beta-Endorphin)
Size:96T
Assay type:Competitive-ELISA
Assay time:2.0h
Sensitivity:9.38 pg/mL
Detection range:15.63-1000 pg/mL
Kit component:
ComponentQuantity (24 Assays)Quantity (96 Assays)Storage
Micro ELISA Plate (Dismountable)8 wells x 3 strips8 wells x 12 strips-20°C, 6 months
Reference Standard1 vial2 vials
Concentrated Biotinylated Detection Ab (100×)1 vial, 60 µL1 vial, 120 µL
Concentrated HRP Conjugate (100×)1 vial, 60 µL1 vial, 120 µL-20°C (shading light), 6 months
Reference Standard & Sample Diluent1 vial, 20 mL1 vial, 20 mL4°C, 6 months
Biotinylated Detection Ab Diluent1 vial, 14 mL1 vial, 14 mL
HRP Conjugate Diluent1 vial, 14 mL1 vial, 14 mL
Concentrated Wash Buffer (25×)1 vial, 30 mL1 vial, 30 mL
Substrate Reagent1 vial, 10 mL1 vial, 10 mL4°C (shading light)
Stop Solution1 vial, 10 mL1 vial, 10 mL4°C
Plate Sealer5 pieces5 pieces
Product Description1 copy1 copy

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with β-EP. During the reaction, β-EP in the sample or standard competes with a fixed amount of β-EP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to β-EP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of β-EP in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Linearity:
Serum (n=5)EDTA plasma (n=5)Cell culture media (n=5)
1:2Range (%)96-11098-11095-105
Average (%)102103100
1:4Range (%)88-9891-10396-112
Average (%)9397102
1:8Range (%)87-10189-10198-110
Average (%)9494104
1:16Range (%)84-9787-10294-109
Average (%)9194102
Recovery:
Sample TypeRange (%)Average Recovery (%)
Serum (n=5)94-10699
EDTA plasma (n=5)84-9991
Cell culture media (n=5)91-10497
Precision:
Intra-assay PrecisionInter-assay Precision
Sample123123
n2020.020.020.020.020.0
Mean (ng/mL)4696.3457.449.297.7488.2
Standard deviation3.23.916.52.74.215.1
C V (%)6.964.053.615.494.33.09
Sample type &Sample volume:serum, plasma and other biological fluids; 50μL
Reproducibility:Both intra-CV and inter-CV are < 10%.
Application:This ELISA kit applies to the in vitro quantitative determination of β-EP concentrations in serum, plasma and other biological fluids.
Specificity:This kit recognizes β-EP in samples. No significant cross-reactivity or interference between β-EP and analogues was observed.

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