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Rat Enolase, Neuron Specific (NSE) ELISA Kit

SKU:
RTDL00765
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P07323
Sensitivity:
0.215ng/mL
Range:
0.625-40ng/mL
ELISA Type:
Sandwich
Synonyms:
ENO2,Enolase 2,Gamma Enolase,2-phospho-D-glycerate hydro-lyase,Neural enolase
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Rat Enolase, Neuron Specific (NSE) ELISA Kit

The Rat Enolase Neuron-Specific (NSE) ELISA Kit is specifically designed for the accurate measurement of NSE levels in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, guaranteeing dependable and consistent results for various research purposes.NSE, also known as gamma-enolase, is an enzyme typically found in neurons and neuroendocrine cells. It is a valuable biomarker for neurological conditions such as Alzheimer's disease, stroke, and traumatic brain injury, making it an essential tool for studying these disorders and investigating potential treatments.

With the Rat Enolase Neuron-Specific (NSE) ELISA Kit, researchers can confidently assess NSE levels in rat samples, advancing their understanding of neurodegenerative diseases and paving the way for innovative therapeutic approaches.

Product Name:Rat Enolase, Neuron Specific (NSE) ELISA Kit
Product Code:RTDL00765
Size:96 Assays
Target:NSE
Synonyms:ENO2, Enolase 2, Gamma Enolase, 2-phospho-D-glycerate hydro-lyase, Neural enolase
Detection Method:Sandwich
Range:0.625-40ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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