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Rat Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) ELISA Kit

SKU:
RTDL00318
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q8R414
Sensitivity:
13.6pg/mL
Range:
31.25-2000pg/mL
ELISA Type:
Sandwich
Synonyms:
VEGF,VEGF-A,VPF,Vascular Endothelial Cell Growth Factor A,Vascular Permeability Factor
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
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Description

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Rat Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) ELISA Kit

The Rat Endocrine Gland-Derived Vascular Endothelial Growth Factor (EG-VEGF) ELISA Kit is a powerful tool for researchers looking to accurately measure EG-VEGF levels in rat serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, ensuring precise and consistent results for a variety of experimental needs.EG-VEGF is a critical factor in angiogenesis, playing a key role in the formation and maintenance of blood vessels. Its implications in various diseases, including cancer and cardiovascular disorders, highlight its importance as a biomarker for disease progression and potential therapeutic interventions.

With the Rat Endocrine Gland-Derived Vascular Endothelial Growth Factor (EG-VEGF) ELISA Kit, researchers can confidently study the role of EG-VEGF in different biological processes and diseases, paving the way for new insights and advancements in the field of vascular biology and medicine.

Product Name:Rat Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF) ELISA Kit
Product Code:RTDL00318
Size:96 Assays
Target:EG-VEGF
Synonyms:VEGF, VEGF-A, VPF, Vascular Endothelial Cell Growth Factor A, Vascular Permeability Factor
Detection Method:Sandwich
Range:31.25-2,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10191
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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