The Rat ELN (Elastin) ELISA Kit is a specialized assay designed for the quantitative detection of Elastin levels in rat biological samples.
Elastin is a crucial component of the extracellular matrix and plays a significant role in providing elasticity and structural integrity to tissues such as skin, lungs, and blood vessels. This ELISA kit offers exceptional sensitivity and specificity, allowing accurate measurement of Elastin concentrations in rat samples. The precise detection of Elastin is essential for research focusing on tissue integrity, vascular health, and connective tissue disorders, providing valuable insights into various physiological and pathological conditions. Manufactured under stringent quality control standards, this ELISA kit ensures robust performance, reliable results, and ease of use, making it an excellent choice for researchers investigating the roles and implications of Elastin in biological systems.
Product Name:
Rat ELN (Elastin) ELISA Kit
SKU:
AEES00288
Target:
Rat ELN (Elastin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat ELN. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat ELN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat ELN, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat ELN. You can calculate the concentration of Rat ELN in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-105
96-113
91-106
Average (%)
98
103
97
1:4
Range (%)
88-102
87-100
83-97
Average (%)
93
93
89
1:8
Range (%)
88-101
85-100
85-100
Average (%)
95
91
92
1:16
Range (%)
93-108
82-93
88-101
Average (%)
99
87
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-107
100
EDTA plasma (n=5)
89-100
94
Cell culture media (n=5)
94-106
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2
3.84
14.43
2.05
4.11
13.92
Standard deviation
0.11
0.2
0.7
0.13
0.2
0.49
C V (%)
5.5
5.21
4.85
6.34
4.87
3.52
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat ELN concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat ELN in samples. No significant cross-reactivity or interference between Rat ELN and analogues was observed.
