CELA3B,Chymotrypsin Like Elastase Family Member 3B,Protease E,Fecal Elastase,Elastase 1,Cholesterol-Binding Pancreatic Protease,Pancreatic Endopeptidase E
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
The Rat Elastase-3B (ELA3B) ELISA Kit is a precision tool for measuring elastase-3B levels in rat serum, plasma, and cell culture supernatants. With its superior sensitivity and specificity, this kit guarantees accurate and consistent results, making it a valuable asset for various research purposes.Elastase-3B, also known as ELA3B, is a key enzyme involved in the breakdown of elastin, a protein that provides elasticity to tissues like blood vessels and skin. Abnormal levels of elastase-3B have been linked to various diseases, including chronic obstructive pulmonary disease (COPD), inflammatory disorders, and cancer.
Therefore, monitoring elastase-3B levels can provide crucial insights into disease progression and potential therapeutic interventions.Invest in the Rat Elastase-3B (ELA3B) ELISA Kit to unlock the mysteries surrounding elastase-3B regulation and its implications in disease pathogenesis. Trust in its high performance and accuracy to advance your research efforts and uncover new opportunities for diagnosis and treatment strategies.
Product Name:
Rat Elastase 3B (ELA3B) ELISA Kit
Product Code:
RTDL00324
Size:
96 Assays
Target:
ELA3B
Synonyms:
CELA3B, Chymotrypsin Like Elastase Family Member 3B, Protease E, Fecal Elastase, Elastase 1, Cholesterol-Binding Pancreatic Protease, Pancreatic Endopeptidase E
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
91-98
94
Heparin plasma (n=5)
90-99
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
