Rat EGF Recombinant Protein (RPPB0172)
- SKU:
- RPPB0172
- Product Type:
- Recombinant Protein
- Species:
- Rat
- Uniprot:
- P07522
- Research Area:
- Growth Factors & Cytokines
Description
| Product Name: | Rat EGF Recombinant Protein |
| Product Code: | RPPB0172 |
| Size: | 100µg |
| Species: | Rat |
| Target: | EGF |
| Synonyms: | Urogastrone, URG, EGF. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | Rat EGF was lyophilized from a 0.2�m filtered concentrated (1.0mg/ml) solution in PBS, pH 7.4. |
| Solubility: | It is recommended to reconstitute the lyophilized Rat EGF in sterile water not less than 100�g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized Rat EGF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Rat EGF should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Purity: | Greater than 98.0% as determined by(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Amino Acid Sequence: | NSNTGCPPSY DGYCLNGGVC MYVESVDRYV CNCVIGYIGE RCQHRDLRWW KLR |
| Biological Activity: | The ED50 as calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells is less than 0.1ng/ml, corresponding to a specific activity of > 10,000,000 units/mg. |
Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide.EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture.
Epidermal Growth Factor Rat Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 53 amino acids and having a molecular mass of 6151 Dalton. The Rat EGF is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | EGF: EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Defects in EGF are the cause of hypomagnesemia type 4 (HOMG4); also known as renal hypomagnesemia normocalciuric. HOMG4 is a disorder characterized by massive renal hypomagnesemia and normal levels of serum calcium and calcium excretion. Clinical features include seizures, mild-to mederate psychomotor retardation, and brisk tendon reflexes. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Ligand, receptor tyrosine kinase; Membrane protein, integral; Hormone Cellular Component: extracellular space; lysosomal membrane; plasma membrane; integral to membrane; intracellular Molecular Function:growth factor activity; calcium ion binding; epidermal growth factor receptor binding Biological Process: epidermal growth factor receptor signaling pathway; peptidyl-tyrosine phosphorylation; positive regulation of mitosis; activation of MAPKK activity; positive regulation of transcription, DNA-dependent; regulation of protein transport; positive regulation of MAP kinase activity; regulation of protein secretion; positive regulation of epidermal growth factor receptor activity; branching morphogenesis of a tube; positive regulation of cell proliferation; negative regulation of secretion; angiogenesis; regulation of peptidyl-tyrosine phosphorylation; positive regulation of phosphorylation; positive regulation of DNA binding; STAT protein nuclear translocation; positive regulation of granule cell precursor proliferation |
| NCBI Summary: | binds the epidermal growth factor receptor; may play a role in MAP kinase mediated signaling pathways [RGD, Feb 2006] |
| UniProt Code: | P07522 |
| NCBI GenInfo Identifier: | 1352360 |
| NCBI Gene ID: | 25313 |
| NCBI Accession: | P07522.2 |
| UniProt Secondary Accession: | P07522,Q63183, |
| UniProt Related Accession: | P07522 |
| Molecular Weight: | 124,126 Da |
| NCBI Full Name: | Pro-epidermal growth factor |
| NCBI Synonym Full Names: | epidermal growth factor |
| NCBI Official Symbol: | Egf�� |
| NCBI Protein Information: | pro-epidermal growth factor |
| UniProt Protein Name: | Pro-epidermal growth factor |
| Protein Family: | EGF-like domain containing protein |
| UniProt Gene Name: | Egf�� |
| UniProt Entry Name: | EGF_RAT |
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