The Rat EGF (Epidermal Growth Factor) ELISA Kit is a specialized assay designed to quantitatively detect the levels of Epidermal Growth Factor in various rat biological samples. Epidermal Growth Factor (EGF) is a critical signaling protein that plays a key role in cell proliferation, differentiation, and survival. It is an essential molecule involved in tissue development, wound healing, and various cellular processes. Accurate measurement of EGF levels using this ELISA kit can provide valuable insights into the role of EGF in cellular growth, tissue repair, and maintenance of epithelial tissues. Dysregulation of EGF has been associated with various pathological conditions, including cancer, inflammatory diseases, and impaired wound healing, making it a crucial target for research in cell biology and regenerative medicine.
The Rat EGF ELISA Kit from Assay Genie offers exceptional sensitivity and specificity, ensuring reliable and reproducible results. Manufactured under strict quality control standards, this assay provides robust performance, delivering accurate quantitative data on EGF levels in biological samples. It is a valuable tool for researchers studying growth factor signaling pathways and their implications in health and disease.
Product Name:
Rat EGF (Epidermal Growth Factor) ELISA Kit
SKU:
AEES00395
Target:
Rat EGF (Epidermal Growth Factor)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat EGF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat EGF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat EGF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat EGF. You can calculate the concentration of Rat EGF in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-112
97-109
86-97
Average (%)
102
103
91
1:4
Range (%)
97-112
84-98
95-108
Average (%)
103
90
101
1:8
Range (%)
97-109
87-100
91-105
Average (%)
103
93
98
1:16
Range (%)
102-116
82-96
98-113
Average (%)
108
88
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-99
92
EDTA plasma (n=5)
93-107
99
Cell culture media (n=5)
93-107
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
55.1
94.4
445.8
57.6
94.6
475.9
Standard deviation
3.4
5.3
15.2
3.2
5.6
19.0
C V (%)
6.17
5.61
3.41
5.56
5.92
3.99
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat EGF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat EGF in samples. No significant cross-reactivity or interference between Rat EGF and analogues was observed.
