The Rat DPP4 (Dipeptidyl Peptidase IV) ELISA Kit is a specialized assay designed to quantitatively measure the levels of Dipeptidyl Peptidase IV in rat biological samples.
Dipeptidyl Peptidase IV, also known as CD26, is a multifunctional cell surface enzyme involved in various biological processes, including glucose homeostasis, immune regulation, and tumor biology. Dipeptidyl Peptidase IV plays a crucial role in glucose metabolism by cleaving incretin hormones such as GLP-1 and GIP, which regulate insulin secretion and blood sugar levels. It is also implicated in immune responses through its interaction with various chemokines and extracellular matrix proteins. Furthermore, DPP4 is associated with cancer progression due to its involvement in tumor cell migration and invasion. Monitoring DPP4 levels through this ELISA kit provides valuable insights into the enzyme's activity and its potential role in metabolic disorders, immune dysregulation, and cancer biology. Accurate quantification of DPP4 in biological samples enables researchers to investigate the enzyme's impact on various physiological and pathological processes. The Rat DPP4 ELISA Kit from Assay Genie offers exceptional sensitivity and specificity, ensuring reliable and reproducible results. Manufactured under stringent quality control standards, this kit provides robust performance and user-friendly protocols, making it a valuable tool for research studies investigating the biological functions and clinical relevance of Dipeptidyl Peptidase IV in different disease settings.
Product Name:
Rat DPP4 (Dipeptidyl Peptldase Ⅳ) ELISA Kit
SKU:
AEES00381
Target:
Rat DPP4 (Dipeptidyl Peptldase Ⅳ)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat DPP4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat DPP4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat DPP4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat DPP4. You can calculate the concentration of Rat DPP4 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-106
92-108
97-113
Average (%)
101
100
105
1:4
Range (%)
88-99
87-101
83-95
Average (%)
93
92
88
1:8
Range (%)
88-101
80-90
84-95
Average (%)
95
86
90
1:16
Range (%)
91-104
81-94
86-100
Average (%)
98
88
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-112
102
EDTA plasma (n=5)
93-106
99
Cell culture media (n=5)
92-106
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.17
5.46
19.19
2.12
5.05
19.92
Standard deviation
0.15
0.25
0.7
0.15
0.21
0.63
C V (%)
6.91
4.58
3.65
7.08
4.16
3.16
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat DPP4 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat DPP4 in samples. No significant cross-reactivity or interference between Rat DPP4 and analogues was observed.
