SLC6A3,DAT1,Sodium-dependent dopamine transporter,Dopamine Active Transporter,SLC6-A3,Solute Carrier Family 6(neurotransmitter Transporter,Dopamine),Member 3
Reactivity:
Rat
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
The Rat Dopamine Transporter (DAT) ELISA Kit is specifically designed for the precise measurement of dopamine transporter levels in rat samples including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and consistent results, making it perfect for a variety of research purposes.The dopamine transporter is a vital protein involved in the reuptake of dopamine in the brain, influencing a variety of neurological processes. Dysregulation of dopamine transporter function has been linked to various neurological disorders such as Parkinson's disease, ADHD, and substance abuse disorders.
Therefore, studying dopamine transporter levels can provide valuable insights into these conditions and aid in the development of potential treatments.Overall, the Rat Dopamine Transporter (DAT) ELISA Kit is an invaluable tool for researchers looking to explore the role of dopamine transporter in neurological disorders and advance our understanding of dopamine-related processes in the brain.
Product Name:
Rat Dopamine Transporter (DAT) ELISA Kit
Product Code:
RTDL00290
Size:
96 Assays
Target:
DAT
Synonyms:
SLC6A3, DAT1, Sodium-dependent dopamine transporter, Dopamine Active Transporter, SLC6-A3, Solute Carrier Family 6(neurotransmitter Transporter,Dopamine),Member 3
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
