The Rat Dopamine Receptor D4 (DRD4) ELISA Kit is specifically designed for the precise and reliable detection of DRD4 levels in rat samples, including serum, plasma, and tissue lysates. With its high sensitivity and specificity, this kit ensures accurate and reproducible results, making it perfect for a variety of research applications.The DRD4 protein is a key receptor involved in the brain's response to dopamine, playing a critical role in various neurological and psychiatric disorders. Understanding DRD4 levels can provide valuable insights into conditions such as ADHD, addiction, and schizophrenia, making this ELISA kit an essential tool for researchers looking to study these disorders and potential therapeutic interventions.
Overall, the Rat Dopamine Receptor D4 (DRD4) ELISA Kit offers researchers a reliable and efficient method for quantifying DRD4 levels in rat samples, making it an invaluable asset for advancing our understanding of neurological and psychiatric disorders.
Product Name:
Rat Dopamine Receptor D4 (DRD4) ELISA Kit
Product Code:
RTDL00310
Size:
96 Assays
Target:
DRD4
Synonyms:
DR-D4, D4R, D4DR, D(2C) dopamine receptor
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
