Rat Des (Desmin) ELISA Kit
- SKU:
- AEES00379
- Product Type:
- ELISA Kit
- ELISA Type:
- Sandwich
- Size:
- 96 Assays
- Reactivity:
- Rat
- Sensitivity:
- 0.19 ng/mL
- Range:
- 0.31-20 ng/mL
- Sample Type:
- Serum, plasma and other biological fluids
Description
Rat Des (Desmin) ELISA Kit
The Rat Desmin (Des) ELISA Kit is designed to quantitatively measure Desmin levels in various biological samples obtained from rat models.
Desmin is a muscle-specific intermediate filament protein that plays a crucial role in maintaining the structural integrity of muscle cells, specifically in skeletal, cardiac, and smooth muscles. Monitoring Desmin levels can offer insights into muscle health, integrity, and potential pathological conditions affecting muscle tissue. Given its muscle-specific nature, changes in Desmin expression levels are often associated with muscle injuries, myopathies, and other muscle-related disorders. Aberrant Desmin levels can indicate muscular damage, degeneration, or regeneration processes, making it a valuable biomarker for assessing muscle health and muscle-related diseases. The Rat Desmin ELISA Kit by Assay Genie provides accurate and precise measurements of Desmin concentrations with exceptional sensitivity and specificity. This kit offers reliable and reproducible results, enabling researchers to study muscle health, muscle-specific diseases, and therapeutic interventions effectively. Manufactured under stringent quality control standards, the kit ensures robust performance and ease of use, making it a valuable tool in muscle biology and related research studies.
| Product Name: | Rat Des (Desmin) ELISA Kit |
| Product Code: | AEES00379 |
| Assay Type: | Sandwich |
| Format: | 96T |
| Assay Time: | 3.5h |
| Reactivity: | Rat |
| Detection Range: | 0.31-20 ng/mL |
| Sensitivity: | 0.19 ng/mL |
| Sample Type & Sample Volume: | Serum, plasma and other biological fluids, 100μL |
| Specificity: | This kit recognizes Rat Des in samples. No significant cross-reactivity or interference between Rat Des and analogues was observed. |
| Reproducibility: | Both intra-CV and inter-CV are < 10%. |
| Application: | This ELISA kit applies to the in vitro quantitative determination of Rat Des concentrations in Serum, plasma and other biological fluids. |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat Des. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat Des and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat Des, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat Des. You can calculate the concentration of Rat Des in the samples by comparing the OD of the samples to the standard curve.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat Des were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat Des were tested on 3 different plates, 20 replicates in each plate.
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 1.01 | 1.83 | 6.92 | 0.98 | 2.01 | 6.57 |
| Standard deviation | 0.05 | 0.08 | 0.30 | 0.07 | 0.09 | 0.23 |
| CV (%) | 4.95 | 4.37 | 4.34 | 7.14 | 4.48 | 3.50 |
Recovery
The recovery of Rat Des spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 90-104 | 96 |
| EDTA plasma (n=8) | 88-102 | 93 |
| Cell culture media (n=8) | 89-101 | 94 |
| Kit Components: | An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
|
| 1. | Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C |
| 2. | Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
| 3. | Aspirate and wash the plate for 3 times |
| 4. | Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
| 5. | Add 90μL Substrate Reagent. Incubate for 15 min at 37°C |
| 6. | Add 50μL Stop Solution |
| 7. | Read the plate at 450nm immediately. Calculation of the results |
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