The Rat Diamine Oxidase (DAO) ELISA Kit is a specialized assay designed for the precise and quantitative detection of Diamine Oxidase levels in various rat biological samples. Diamine Oxidase is an enzyme that plays a crucial role in the metabolism of histamine and other biogenic amines. As an important element in the gastrointestinal tract, Diamine Oxidase contributes to the detoxification and regulation of histamine levels, influencing various physiological functions. Accurate measurement of Diamine Oxidase with this ELISA kit provides researchers with valuable insights into the enzyme's activity in histamine clearance, inflammatory responses, and gastrointestinal health.
By offering exceptional sensitivity and specificity, this kit enables detailed analysis of Diamine Oxidase levels, facilitating investigations into the enzyme's involvement in allergic reactions, gastrointestinal disorders, and immune responses. The Rat DAO ELISA Kit by Assay Genie delivers reliable and reproducible results, empowering researchers to explore the intricate role of Diamine Oxidase across different biological contexts. Manufactured under stringent quality control standards, this kit ensures robust performance and user-friendly protocols, making it an excellent tool for research studies focused on enzymatic activity, histamine regulation, and gastrointestinal physiology.
Product Name:
Rat DAO (Diamine Oxidase) ELISA Kit
SKU:
AEES00529
Target:
Rat DAO (Diamine Oxidase)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat DAO. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat DAO and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat DAO, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat DAO. You can calculate the concentration of Rat DAO in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-101
86-99
97-106
Average (%)
96
94
101
1:4
Range (%)
87-101
87-97
88-100
Average (%)
93
93
92
1:8
Range (%)
91-103
91-105
87-96
Average (%)
95
99
93
1:16
Range (%)
98-105
96-105
88-98
Average (%)
102
99
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
102-113
105
EDTA plasma (n=5)
84-96
92
Cell culture media (n=5)
86-100
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.46
1.19
4.05
0.45
1.02
4.91
Standard deviation
0.02
0.06
0.2
0.03
0.05
0.23
C V (%)
4.35
5.04
4.94
6.67
4.9
4.68
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat DAO concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat DAO in samples. No significant cross-reactivity or interference between Rat DAO and analogues was observed.
