The Rat D-Lactate Dehydrogenase (D-LDH) ELISA Kit is a specialized enzyme-linked immunosorbent assay designed to quantitatively measure D-Lactate Dehydrogenase levels in rat biological samples.
D-Lactate Dehydrogenase is an enzyme that plays a crucial role in the metabolism of D-lactate, a byproduct of bacterial fermentation in the intestinal tract and a potential biomarker for intestinal health and injury. D-Lactate Dehydrogenase is involved in the conversion of D-lactate to pyruvate, participating in the metabolic pathways that regulate intestinal bacterial fermentation. Monitoring D-Lactate Dehydrogenase levels provides insights into D-lactate metabolism and its potential implications in gastrointestinal health and dysbiosis. This ELISA kit enables researchers to accurately quantify D-Lactate Dehydrogenase levels in biological samples, facilitating investigations into the enzyme's activity and its correlation with intestinal health, bacterial overgrowth, and gut dysbiosis. Understanding the role of D-Lactate Dehydrogenase can shed light on gastrointestinal disorders and help develop targeted therapeutic strategies for maintaining intestinal homeostasis. The Rat D-LDH ELISA Kit from Assay Genie offers exceptional sensitivity and specificity, ensuring reliable and reproducible results. Manufactured under stringent quality control standards, this kit provides robust performance and user-friendly protocols, making it a valuable tool for research studies exploring the link between D-Lactate Dehydrogenase activity and intestinal health in rat models.
Product Name:
Rat D-LDH (D-Lactate Dehydrogenase) ELISA Kit
SKU:
AEES00382
Target:
Rat D-LDH (D-Lactate Dehydrogenase)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 pmol/mL
Detection range:
0.16-10 pmol/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat D-LDH. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat D-LDH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat D-LDH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat D-LDH. You can calculate the concentration of Rat D-LDH in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-96
96-111
92-104
Average (%)
91
102
98
1:4
Range (%)
95-108
88-103
95-109
Average (%)
102
94
101
1:8
Range (%)
96-110
82-94
95-109
Average (%)
102
89
101
1:16
Range (%)
99-112
81-93
91-108
Average (%)
105
88
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-104
99
EDTA plasma (n=5)
93-107
99
Cell culture media (n=5)
86-99
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.5
1.2
3.6
0.5
1.1
3.6
Standard deviation
0.03
0.07
0.13
0.04
0.06
0.19
C V (%)
6
5.83
3.61
8.0
5.45
5.28
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat D-LDH concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat D-LDH in samples. No significant cross-reactivity or interference between Rat D-LDH and analogues was observed.
