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Rat Cytokine Receptor Like Factor 1 (CRLF1) ELISA Kit

SKU:
RTDL00253
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
D3ZIV9
Sensitivity:
0.063ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
CISS,CISS1,CLF,CLF-1,NR6,Cytokine-like factor 1,ZcytoR5
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€749
Frequently bought together:

Description

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Rat Cytokine Receptor Like Factor 1 (CRLF1) ELISA Kit

The Rat Cytokine Receptor-Like Factor 1 (CRLF1) ELISA Kit is a highly sensitive and specific assay designed for the accurate detection of CRLF1 levels in rat serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it perfect for a wide range of research applications.CRLF1 is a cytokine receptor-like factor that plays a crucial role in the regulation of immune responses and cell proliferation. It is implicated in various inflammatory and autoimmune diseases, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.

With its advanced technology and optimized protocols, the Rat CRLF1 ELISA Kit offers researchers a powerful tool for investigating the role of CRLF1 in disease pathogenesis and developing novel treatments. Order now to accelerate your research and advance scientific knowledge in the field of cytokine biology.

Product Name:Rat Cytokine Receptor Like Factor 1 (CRLF1) ELISA Kit
Product Code:RTDL00253
Size:96 Assays
Target:CRLF1
Synonyms:CISS, CISS1, CLF, CLF-1, NR6, Cytokine-like factor 1, ZcytoR5
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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