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Rat Cytochrome P450 2C19 (CYP2C19) ELISA Kit

SKU:
RTDL00281
Product Type:
ELISA Kit
Sensitivity:
0.29ng/mL
Range:
0.781-50ng/mL
ELISA Type:
Sandwich
Synonyms:
CPCJ,CYP2C,CYPIIC17,CYPIIC19,P450C2C,Cytochrome P450-11A,Cytochrome P450-254C,Mephenytoin 4-hydroxylase,limonene 6-monooxygenase
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€749
Frequently bought together:

Description

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Rat Cytochrome P450 2C19 (CYP2C19) ELISA Kit

The Rat Cytochrome P450 2C19 (CYP2C19) ELISA Kit is specifically designed for the accurate quantification of CYP2C19 levels in rat samples. This kit offers high sensitivity and specificity, allowing for precise and reliable results in serum, plasma, and cell culture supernatants.Cytochrome P450 2C19 is a key enzyme involved in drug metabolism and plays a crucial role in the activation and detoxification of various pharmaceutical compounds.

Understanding the expression levels of CYP2C19 in rats is essential for evaluating drug efficacy, toxicity, and potential drug-drug interactions in preclinical studies.With its ease of use and ability to deliver consistent results, the Rat Cytochrome P450 2C19 (CYP2C19) ELISA Kit is an indispensable tool for researchers studying drug metabolism and exploring novel therapeutic interventions in animal models.

Product Name:Rat Cytochrome P450 2C19 (CYP2C19) ELISA Kit
Product Code:RTDL00281
Size:96 Assays
Target:CYP2C19
Synonyms:CPCJ, CYP2C, CYPIIC17, CYPIIC19, P450C2C, Cytochrome P450-11A, Cytochrome P450-254C, Mephenytoin 4-hydroxylase, limonene 6-monooxygenase
Detection Method:Sandwich
Range:0.781-50ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-9894
Heparin plasma (n=5)89-10295
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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