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Rat Cytochrome P450 11A1 (CYP11A1) ELISA Kit

SKU:
RTDL00277
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P14137
Sensitivity:
0.064ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
CYP11A,P450SCC,Cholesterol desmolase,Cholesterol Side Chain Cleavage Enzyme,Cytochrome P450 Family 11 Subfamily A Polypeptide 1
Reactivity:
Rat
Sample Type:
Tissue Homogenates And Other Biological Fluids
€749
Frequently bought together:

Description

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Rat Cytochrome P450 11A1 (CYP11A1) ELISA Kit

The Rat Cytochrome P450 11A1 (CYP11A1) ELISA Kit is specifically designed for the accurate measurement of CYP11A1 levels in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results that are suitable for a variety of research applications.CYP11A1 is a key enzyme involved in the biosynthesis of steroid hormones, crucial for the regulation of various physiological processes such as metabolism and reproduction.

Dysregulation of CYP11A1 has been linked to disorders such as adrenal insufficiency and hormonal imbalance, making it a valuable biomarker for studying these conditions and developing potential treatments.With the Rat Cytochrome P450 11A1 (CYP11A1) ELISA Kit, researchers can accurately assess CYP11A1 levels in rat samples, providing valuable insights into steroid hormone metabolism and potential therapeutic targets for related disorders.

Product Name:Rat Cytochrome P450 11A1 (CYP11A1) ELISA Kit
Product Code:RTDL00277
Size:96 Assays
Target:CYP11A1
Synonyms:CYP11A, P450SCC, Cholesterol desmolase, Cholesterol Side Chain Cleavage Enzyme, Cytochrome P450 Family 11 Subfamily A Polypeptide 1
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10191
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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