The Rat Cys-C (Cystatin C) ELISA Kit is a specialized assay designed for the quantitative determination of Cystatin C levels in various rat biological samples. Cystatin C, a low-molecular-weight protein, serves as a potent inhibitor of cysteine proteases and is primarily secreted by nucleated cells, with notable expression in tissues like the brain, liver, and spleen. As a biomarker of kidney function, Cystatin C has garnered significant attention for its potential in assessing glomerular filtration rate (GFR) and monitoring renal health. With its critical role in renal function, Cystatin C has been recognized as a reliable marker for kidney injury and chronic kidney disease (CKD) progression.
Elevated levels of Cystatin C are associated with impaired renal function, making it a valuable indicator for diagnosing and monitoring kidney-related disorders. The quantitative measurement of Cystatin C in biological samples enables researchers to evaluate kidney function and renal health status, providing valuable insights into disease progression and treatment response. The Rat Cys-C ELISA Kit offers exceptional sensitivity and specificity, allowing for accurate and reproducible results. Manufactured under stringent quality control standards, this ELISA kit ensures robust performance and ease of use, making it a preferred tool for researchers and clinicians studying kidney diseases and related conditions.
Product Name:
Rat Cys-C (Cystatin C) ELISA Kit
SKU:
AEES00374
Target:
Rat Cys-C (Cystatin C)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat Cys-C. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat Cys-C and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat Cys-C, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat Cys-C. You can calculate the concentration of Rat Cys-C in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-107
90-105
85-98
Average (%)
100
97
90
1:4
Range (%)
99-111
84-97
93-105
Average (%)
104
90
100
1:8
Range (%)
97-111
83-95
94-108
Average (%)
104
87
101
1:16
Range (%)
98-113
81-96
96-109
Average (%)
104
88
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-104
96
EDTA plasma (n=5)
86-99
91
Cell culture media (n=5)
93-107
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.5
1.1
4.0
0.5
1.1
4.2
Standard deviation
0.03
0.05
0.2
0.03
0.06
0.15
C V (%)
6
4.55
5.0
6.0
5.45
3.57
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat Cys-C concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat Cys-C in samples. No significant cross-reactivity or interference between Rat Cys-C and analogues was observed.
