The Rat CypA (Cyclophilin A) CLIA Kit is specifically designed for the quantitative detection of cyclophilin A levels in rat samples, including serum, plasma, and tissue homogenates. This kit offers high sensitivity and accuracy, providing reliable and reproducible results for various research applications.Cyclophilin A is a ubiquitous protein known for its role in protein folding and trafficking, as well as its involvement in immune responses and inflammation.
Dysregulation of cyclophilin A has been linked to various diseases, including infectious diseases, autoimmune disorders, and cancer. Therefore, the Rat CypA CLIA Kit serves as a valuable tool for studying the role of cyclophilin A in these conditions and investigating potential therapeutic interventions.
Product Name:
Rat CYPA (Cyclophilin A) CLIA Kit
SKU:
RTES00165
Target:
Rat CYPA (Cyclophilin A)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.56 ng/mL
Detection range:
0.94-60 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat CYPA. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CYPA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CYPA, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat CYPA. You can calculate the concentration of Rat CYPA in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-108
83-96
84-99
Average (%)
101
90
91
1:4
Range (%)
84-97
97-112
94-107
Average (%)
91
104
100
1:8
Range (%)
100-116
88-98
91-107
Average (%)
106
93
98
1:16
Range (%)
91-102
100-116
97-113
Average (%)
97
106
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-110
104
EDTA plasma (n=5)
86-97
91
Cell culture media (n=5)
93-108
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.85
9.63
20.47
2.71
9.53
19.7
Standard deviation
0.31
0.86
1.56
0.25
0.86
2.13
C V (%)
10.88
8.93
7.62
9.23
9.02
10.81
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat CYPA concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat CYPA in samples. No significant cross-reactivity or interference between Rat CYPA and analogues was observed.
