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Rat Cyclin D1 (CCND1) ELISA Kit

SKU:
RTDL00188
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P39948
Sensitivity:
0.057ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
CCN-D1,BCL1,PRAD1,U21B31,G1/S-Specific Cyclin-D1,Parathyroid Adenomatosis 1,B-Cell CLL/Lymphoma 1,G1/S-Specific Cyclin D1,PRAD1 oncogene
Reactivity:
Rat
Sample Type:
Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
Frequently bought together:

Description

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Rat Cyclin D1 (CCND1) ELISA Kit

The Rat Cyclin D1 (CCND1) ELISA Kit is a highly reliable and sensitive assay designed for the accurate quantitative measurement of Cyclin D1 levels in rat serum, plasma, and cell culture supernatants. This kit provides precise and reproducible results, making it an essential tool for research applications in the fields of cancer, cell cycle regulation, and cell proliferation.Cyclin D1 is a key regulator of cell cycle progression, influencing cell growth and division.

Dysregulation of Cyclin D1 has been implicated in various diseases, including cancer and developmental disorders, making it an important target for further investigation and therapeutic development. With its high specificity and ease of use, the Rat Cyclin D1 (CCND1) ELISA Kit is a valuable resource for researchers seeking to study the role of Cyclin D1 in cellular processes and disease mechanisms in rat models.

Product Name:Rat Cyclin D1 (CCND1) ELISA Kit
Product Code:RTDL00188
Size:96 Assays
Target:CCND1
Synonyms:CCN-D1, BCL1, PRAD1, U21B31, G1/S-Specific Cyclin-D1, Parathyroid Adenomatosis 1, B-Cell CLL/Lymphoma 1, G1/S-Specific Cyclin D1, PRAD1 oncogene
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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