Rat Cyclic AMP-responsive element-binding protein 1 (Creb1) ELISA Kit (RTEB0869)
- SKU:
- RTEB0869
- Product Type:
- ELISA Kit
- Size:
- 96 Assays
- Uniprot:
- P15337
- Range:
- 0.312-20 ng/mL
- ELISA Type:
- Sandwich
- Synonyms:
- CREB, active transcription factor CREB, cAMP responsive element binding protein 1
- Reactivity:
- Rat
Description
Rat Cyclic AMP-responsive element-binding protein 1 (Creb1) ELISA Kit
The Rat CREB1 (Cyclic AMP Responsive Element Binding Protein 1) ELISA Kit is a powerful tool for the precise measurement of CREB1 levels in rat samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, delivering accurate and reproducible results for a variety of research applications.CREB1 is a key transcription factor that plays a critical role in regulating gene expression in response to cyclic AMP (cAMP) signaling pathways. It is involved in various cellular processes such as neuronal plasticity, memory formation, and metabolism.
Dysregulation of CREB1 has been linked to neurological disorders, learning and memory deficits, and psychiatric conditions, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.Overall, the Rat CREB1 ELISA Kit is an invaluable tool for researchers investigating the role of CREB1 in physiological and pathological processes, offering reliable and precise measurement of CREB1 levels in rat samples.
Product Name: | Rat Cyclic AMP-responsive element-binding protein 1 (Creb1) ELISA Kit |
SKU: | RTEB0869 |
Size: | 96T |
Target: | Rat Cyclic AMP-responsive element-binding protein 1 (Creb1) |
Synonyms: | CREB-1, Creb-1 |
Assay Type: | Sandwich |
Detection Method: | ELISA |
Reactivity: | Rat |
Detection Range: | 0.312-20ng/mL |
Sensitivity: | 0.185ng/mL |
Intra CV: | 5.9% | ||||||||||||||||||||
Inter CV: | 8.3% | ||||||||||||||||||||
Linearity: |
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Recovery: |
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Function: | Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-117 phosphorylation. Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells. |
Uniprot: | P15337 |
Sample Type: | Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids |
Specificity: | Natural and recombinant rat Cyclic AMP-responsive element-binding protein 1 |
Sub Unit: | Interacts with PPRC1. Binds DNA as a dimer. Interacts, through the bZIP domain, with the coactivators TORC1/CRTC1, TORC2/CRTC2 and TORC3/CRTC3 (By similarity). When phosphorylated on Ser-133, binds CREBBP. Interacts with ARRB1. Interacts (phosphorylated form) with TOX3. Binds to HIPK2 (By similarity). Interacts with SGK1 (By similarity). Interacts with CREBL2; regulates CREB1 phosphorylation, stability and transcriptional activity. |
Research Area: | Neurosciences |
Subcellular Location: | Nucleus |
Storage: | Please see kit components below for exact storage details |
Note: | For research use only |
UniProt Protein Function: | CREB: a transcription factor of the leucine zipper family of DNA binding proteins. Binds as a homodimer to the cAMP-responsive element (CRE). Phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Two splice-variant isoforms have been described. |
UniProt Protein Details: | Protein type:DNA-binding; Transcription factor Cellular Component: axon; chromatin; mitochondrial matrix; mitochondrion; nuclear chromatin; nucleoplasm; nucleus; transcription factor complex Molecular Function:DNA binding; double-stranded DNA binding; enzyme binding; histone acetyltransferase binding; Hsp70 protein binding; identical protein binding; protein binding; RNA polymerase II transcription factor activity, enhancer binding; sequence-specific DNA binding; transcription factor activity; transcription factor binding Biological Process: aging; axonogenesis; circadian rhythm; lactation; mammary gland development; memory; pituitary gland development; positive regulation of apoptosis; positive regulation of fat cell differentiation; positive regulation of hormone secretion; positive regulation of lipid biosynthetic process; positive regulation of multicellular organism growth; positive regulation of osteoclast differentiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; positive regulation of transcriptional preinitiation complex assembly; positive regulation of transforming growth factor-beta3 production; protein amino acid phosphorylation; protein stabilization; regulation of apoptosis; regulation of cell size; regulation of circadian rhythm; regulation of fibroblast proliferation; regulation of transcription, DNA-dependent; response to activity; response to drug; response to glucagon stimulus; response to hypoxia; response to nicotine; response to organic substance; secretory granule organization and biogenesis; transcription from RNA polymerase II promoter; transforming growth factor beta receptor signaling pathway; visual learning |
NCBI Summary: | binds the cAMP response element in many gene promoters and regulates transcription [RGD, Feb 2006] |
UniProt Code: | P15337 |
NCBI GenInfo Identifier: | 117435 |
NCBI Gene ID: | 81646 |
NCBI Accession: | P15337.1 |
UniProt Related Accession: | P15337 |
Molecular Weight: | 35,081 Da |
NCBI Full Name: | Cyclic AMP-responsive element-binding protein 1 |
NCBI Synonym Full Names: | cAMP responsive element binding protein 1 |
NCBI Official Symbol: | Creb1 |
NCBI Official Synonym Symbols: | Creb |
NCBI Protein Information: | cyclic AMP-responsive element-binding protein 1 |
UniProt Protein Name: | Cyclic AMP-responsive element-binding protein 1 |
Protein Family: | Cyclic AMP-responsive element-binding protein |
UniProt Gene Name: | Creb1 |
UniProt Entry Name: | CREB1_RAT |
Component | Quantity (96 Assays) | Storage |
ELISA Microplate (Dismountable) | 8×12 strips | -20°C |
Lyophilized Standard | 2 | -20°C |
Sample Diluent | 20ml | -20°C |
Assay Diluent A | 10mL | -20°C |
Assay Diluent B | 10mL | -20°C |
Detection Reagent A | 120µL | -20°C |
Detection Reagent B | 120µL | -20°C |
Wash Buffer | 30mL | 4°C |
Substrate | 10mL | 4°C |
Stop Solution | 10mL | 4°C |
Plate Sealer | 5 | - |
Other materials and equipment required:
- Microplate reader with 450 nm wavelength filter
- Multichannel Pipette, Pipette, microcentrifuge tubes and disposable pipette tips
- Incubator
- Deionized or distilled water
- Absorbent paper
- Buffer resevoir
*Note: The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Allow all reagents to reach room temperature (Please do not dissolve the reagents at 37°C directly). All the reagents should be mixed thoroughly by gently swirling before pipetting. Avoid foaming. Keep appropriate numbers of strips for 1 experiment and remove extra strips from microtiter plate. Removed strips should be resealed and stored at -20°C until the kits expiry date. Prepare all reagents, working standards and samples as directed in the previous sections. Please predict the concentration before assaying. If values for these are not within the range of the standard curve, users must determine the optimal sample dilutions for their experiments. We recommend running all samples in duplicate.
Step | |
1. | Add Sample: Add 100µL of Standard, Blank, or Sample per well. The blank well is added with Sample diluent. Solutions are added to the bottom of micro ELISA plate well, avoid inside wall touching and foaming as possible. Mix it gently. Cover the plate with sealer we provided. Incubate for 120 minutes at 37°C. |
2. | Remove the liquid from each well, don't wash. Add 100µL of Detection Reagent A working solution to each well. Cover with the Plate sealer. Gently tap the plate to ensure thorough mixing. Incubate for 1 hour at 37°C. Note: if Detection Reagent A appears cloudy warm to room temperature until solution is uniform. |
3. | Aspirate each well and wash, repeating the process three times. Wash by filling each well with Wash Buffer (approximately 400µL) (a squirt bottle, multi-channel pipette,manifold dispenser or automated washer are needed). Complete removal of liquid at each step is essential. After the last wash, completely remove remaining Wash Buffer by aspirating or decanting. Invert the plate and pat it against thick clean absorbent paper. |
4. | Add 100µL of Detection Reagent B working solution to each well. Cover with the Plate sealer. Incubate for 60 minutes at 37°C. |
5. | Repeat the wash process for five times as conducted in step 3. |
6. | Add 90µL of Substrate Solution to each well. Cover with a new Plate sealer and incubate for 10-20 minutes at 37°C. Protect the plate from light. The reaction time can be shortened or extended according to the actual color change, but this should not exceed more than 30 minutes. When apparent gradient appears in standard wells, user should terminatethe reaction. |
7. | Add 50µL of Stop Solution to each well. If color change does not appear uniform, gently tap the plate to ensure thorough mixing. |
8. | Determine the optical density (OD value) of each well at once, using a micro-plate reader set to 450 nm. User should open the micro-plate reader in advance, preheat the instrument, and set the testing parameters. |
9. | After experiment, store all reagents according to the specified storage temperature respectively until their expiry. |
When carrying out an ELISA assay it is important to prepare your samples in order to achieve the best possible results. Below we have a list of procedures for the preparation of samples for different sample types.
Sample Type | Protocol |
Serum | If using serum separator tubes, allow samples to clot for 30 minutes at room temperature. Centrifuge for 10 minutes at 1,000x g. Collect the serum fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. If serum separator tubes are not being used, allow samples to clot overnight at 2-8°C. Centrifuge for 10 minutes at 1,000x g. Remove serum and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. |
Plasma | Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples at 4°C for 15 mins at 1000 × g within 30 mins of collection. Collect the plasma fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. Note: Over haemolysed samples are not suitable for use with this kit. |
Urine & Cerebrospinal Fluid | Collect the urine (mid-stream) in a sterile container, centrifuge for 20 mins at 2000-3000 rpm. Remove supernatant and assay immediately. If any precipitation is detected, repeat the centrifugation step. A similar protocol can be used for cerebrospinal fluid. |
Cell culture supernatant | Collect the cell culture media by pipette, followed by centrifugation at 4°C for 20 mins at 1500 rpm. Collect the clear supernatant and assay immediately. |
Cell lysates | Solubilize cells in lysis buffer and allow to sit on ice for 30 minutes. Centrifuge tubes at 14,000 x g for 5 minutes to remove insoluble material. Aliquot the supernatant into a new tube and discard the remaining whole cell extract. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C. |
Tissue homogenates | The preparation of tissue homogenates will vary depending upon tissue type. Rinse tissue with 1X PBS to remove excess blood & homogenize in 20ml of 1X PBS (including protease inhibitors) and store overnight at ≤ -20°C. Two freeze-thaw cycles are required to break the cell membranes. To further disrupt the cell membranes you can sonicate the samples. Centrifuge homogenates for 5 mins at 5000xg. Remove the supernatant and assay immediately or aliquot and store at -20°C or -80°C. |
Tissue lysates | Rinse tissue with PBS, cut into 1-2 mm pieces, and homogenize with a tissue homogenizer in PBS. Add an equal volume of RIPA buffer containing protease inhibitors and lyse tissues at room temperature for 30 minutes with gentle agitation. Centrifuge to remove debris. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C. |
Breast Milk | Collect milk samples and centrifuge at 10,000 x g for 60 min at 4°C. Aliquot the supernatant and assay. For long term use, store samples at -80°C. Minimize freeze/thaw cycles. |