Rat CXCL16 (Chemokine C-X-C-Motif Ligand 16) ELISA Kit
- SKU:
- AEES00349
- Product Type:
- ELISA Kit
- ELISA Type:
- Sandwich
- Size:
- 96 Assays
- Reactivity:
- Rat
- Sensitivity:
- 0.10 ng/mL
- Range:
- 0.16-10 ng/mL
- Sample Type:
- Serum, plasma and other biological fluids
Description
Rat CXCL16 (Chemokine C-X-C-Motif Ligand 16) ELISA Kit
The Rat CXCL16 (Chemokine C-X-C-Motif Ligand 16) ELISA Kit is a specialized assay designed to quantitatively measure the levels of CXCL16 in rat biological samples. CXCL16 is a unique chemokine that plays a crucial role in regulating immune responses, cell migration, and inflammation. This ELISA kit enables researchers to accurately assess the concentrations of CXCL16, providing valuable insights into its involvement in various cellular processes and immune functions. By detecting and quantifying CXCL16, researchers can gain a deeper understanding of its impact on inflammatory pathways, immune cell recruitment, and disease pathogenesis. The quantitative data generated by this ELISA kit can aid in elucidating the role of CXCL16 in various health conditions, autoimmune disorders, and inflammatory diseases.
Accurate measurement of CXCL16 levels is essential for unraveling its complex functions and potential therapeutic implications. The Rat CXCL16 ELISA Kit offered by Assay Genie ensures precise and reproducible results with exceptional sensitivity and specificity. Manufactured under stringent quality control measures, this kit delivers reliable performance and user-friendly protocols, making it a valuable tool for research investigations into the role of Chemokine C-X-C-Motif Ligand 16 in diverse biological processes and disease conditions.
| Product Name: | Rat CXCL16 (Chemokine C-X-C-Motif Ligand 16) ELISA Kit |
| Product Code: | AEES00349 |
| Assay Type: | Sandwich |
| Format: | 96T |
| Assay Time: | 3.5h |
| Reactivity: | Rat |
| Detection Range: | 0.16-10 ng/mL |
| Sensitivity: | 0.10 ng/mL |
| Sample Type & Sample Volume: | Serum, plasma and other biological fluids, 100μL |
| Specificity: | This kit recognizes Rat CXCL16 in samples. No significant cross-reactivity or interference between Rat CXCL16 and analogues was observed. |
| Reproducibility: | Both intra-CV and inter-CV are < 10%. |
| Application: | This ELISA kit applies to the in vitro quantitative determination of Rat CXCL16 concentrations in Serum, plasma and other biological fluids. |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat CXCL16. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CXCL16 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CXCL16, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat CXCL16. You can calculate the concentration of Rat CXCL16 in the samples by comparing the OD of the samples to the standard curve.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat CXCL16 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat CXCL16 were tested on 3 different plates, 20 replicates in each plate.
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 0.50 | 1.10 | 3.60 | 0.50 | 1.00 | 3.60 |
| Standard deviation | 0.03 | 0.06 | 0.15 | 0.03 | 0.05 | 0.15 |
| CV (%) | 6.00 | 5.45 | 4.17 | 6.00 | 5.00 | 4.17 |
Recovery
The recovery of Rat CXCL16 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 89-100 | 95 |
| EDTA plasma (n=8) | 94-110 | 102 |
| Cell culture media (n=8) | 86-98 | 93 |
| Kit Components: | An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
|
| 1. | Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C |
| 2. | Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
| 3. | Aspirate and wash the plate for 3 times |
| 4. | Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
| 5. | Add 90μL Substrate Reagent. Incubate for 15 min at 37°C |
| 6. | Add 50μL Stop Solution |
| 7. | Read the plate at 450nm immediately. Calculation of the results |
Related Products
Rat Chemokine C-X-C-Motif Ligand 16 (CXCL16) ELISA Kit
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Rat C-X-C motif chemokine 16 (Cxcl16) ELISA Kit (RTEB0446)
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