Rat CX3CL1 (Chemokine C-X3-C-Motif Ligand 1) ELISA Kit
- SKU:
- AEES00400
- Product Type:
- ELISA Kit
- ELISA Type:
- Sandwich
- Size:
- 96 Assays
- Reactivity:
- Rat
- Sensitivity:
- 0.10 ng/mL
- Range:
- 0.16-10 ng/mL
- Sample Type:
- Serum, plasma and other biological fluids
Description
Rat CX3CL1 (Chemokine C-X3-C-Motif Ligand 1) ELISA Kit
The Rat CX3CL1 (Chemokine C-X3-C-Motif Ligand 1) ELISA Kit is designed to quantitatively measure the levels of CX3CL1 in various rat biological samples. CX3CL1, also known as Fractalkine, is a unique chemokine that exists in both a membrane-bound form and a soluble form. It plays a critical role as a chemoattractant for leukocytes and is involved in mediating cell adhesion and migration in processes related to inflammation and immune response. Accurate quantification of CX3CL1 levels using this ELISA kit is vital for studying its role in various physiological and pathophysiological processes, including inflammatory responses, immune cell recruitment, and neurological functions.
CX3CL1 has been implicated in conditions such as neuroinflammation, immune cell trafficking, and vascular dysfunction, making it a valuable target for research in neuroimmunology, cardiovascular biology, and inflammatory diseases. The Rat CX3CL1 ELISA Kit offered by Assay Genie delivers exceptional sensitivity and specificity, ensuring dependable and reproducible results. Manufactured under stringent quality standards, this kit provides robust performance and ease of use, making it an ideal choice for researchers conducting investigations into the multifaceted roles of CX3CL1 in biological systems.
| Product Name: | Rat CX3CL1 (Chemokine C-X3-C-Motif Ligand 1) ELISA Kit |
| Product Code: | AEES00400 |
| Assay Type: | Sandwich |
| Format: | 96T |
| Assay Time: | 3.5h |
| Reactivity: | Rat |
| Detection Range: | 0.16-10 ng/mL |
| Sensitivity: | 0.10 ng/mL |
| Sample Type & Sample Volume: | Serum, plasma and other biological fluids, 100μL |
| Specificity: | This kit recognizes Rat CX3CL1 in samples. No significant cross-reactivity or interference between Rat CX3CL1 and analogues was observed. |
| Reproducibility: | Both intra-CV and inter-CV are < 10%. |
| Application: | This ELISA kit applies to the in vitro quantitative determination of Rat CX3CL1 concentrations in Serum, plasma and other biological fluids. |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat CX3CL1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CX3CL1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CX3CL1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat CX3CL1. You can calculate the concentration of Rat CX3CL1 in the samples by comparing the OD of the samples to the standard curve.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat CX3CL1 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat CX3CL1 were tested on 3 different plates, 20 replicates in each plate.
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 0.48 | 1.17 | 4.16 | 0.45 | 1.26 | 4.33 |
| Standard deviation | 0.03 | 0.06 | 0.13 | 0.03 | 0.06 | 0.23 |
| CV (%) | 6.25 | 5.13 | 3.13 | 6.67 | 4.76 | 5.31 |
Recovery
The recovery of Rat CX3CL1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 93-108 | 99 |
| EDTA plasma (n=8) | 85-100 | 92 |
| Cell culture media (n=8) | 83-95 | 90 |
| Kit Components: | An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
|
| 1. | Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C |
| 2. | Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
| 3. | Aspirate and wash the plate for 3 times |
| 4. | Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
| 5. | Add 90μL Substrate Reagent. Incubate for 15 min at 37°C |
| 6. | Add 50μL Stop Solution |
| 7. | Read the plate at 450nm immediately. Calculation of the results |
Related Products
Rat Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) ELISA Kit
Human Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) ELISA Kit
Mouse Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) ELISA Kit
Human CX3CL1 (Chemokine C-X3-C-Motif Ligand 1) ELISA Kit (HUES01331)
