Based on the information provided in the URL, we present to you the Rat CVL Cytovillin CLIA Kit (RTES00173). This innovative kit is specifically designed for the precise and reliable detection of Cytovillin levels in rat cell culture supernatants.Cytovillin is a key cytoskeletal protein that plays a critical role in cell adhesion and migration. It is essential for cell shape maintenance and intracellular signaling processes. Abnormal levels of Cytovillin have been associated with various diseases and disorders, making it a valuable biomarker for research and potential therapeutic interventions.
The Rat CVL Cytovillin CLIA Kit offers high sensitivity and specificity, ensuring accurate and reproducible results for a wide range of research applications. With this kit, researchers can confidently study the role of Cytovillin in physiological processes and disease pathogenesis.
Product Name:
Rat CVL (Cytovillin) CLIA Kit
SKU:
RTES00173
Target:
Rat CVL (Cytovillin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat CVL. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CVL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CVL, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat CVL. You can calculate the concentration of Rat CVL in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
103-117
96-111
85-98
Average (%)
110
103
91
1:4
Range (%)
88-100
99-116
90-105
Average (%)
93
106
96
1:8
Range (%)
98-113
91-105
99-114
Average (%)
104
99
107
1:16
Range (%)
90-103
89-104
87-101
Average (%)
95
96
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-113
103
EDTA plasma (n=5)
86-99
91
Cell culture media (n=5)
100-114
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
26.53
44.76
199.4
26.35
44.62
211.54
Standard deviation
2.54
3.57
15.17
3.3
4.14
17.41
C V (%)
9.57
7.98
7.61
12.52
9.28
8.23
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat CVL concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat CVL in samples. No significant cross-reactivity or interference between Rat CVL and analogues was observed.
