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Rat CTSD (Cathepsin D) ELISA Kit

SKU:
AEES00346
Product Type:
ELISA Kit
ELISA Type:
Sandwich
Size:
96 Assays
Reactivity:
Rat
Sensitivity:
9.38 pg/mL
Range:
15.63-1000 pg/mL
Sample Type:
Serum, plasma and other biological fluids
€499
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Description

Rat CTSD (Cathepsin D) ELISA Kit

The Rat CTSD (Cathepsin D) ELISA Kit is a valuable tool designed for the quantitative detection of Cathepsin D levels in rat biological samples. Cathepsin D is a critical lysosomal protease involved in various cellular functions, including protein processing, degradation, and turnover. It plays a pivotal role in intracellular proteolysis, autophagy, apoptosis, and cell proliferation, highlighting its significance in cellular homeostasis and pathophysiological processes. Accurate measurement of Cathepsin D levels is essential for studying its role in physiological conditions, such as cellular metabolism and protein turnover, as well as in pathological conditions, including cancer, neurodegenerative diseases, and lysosomal storage disorders.

The ELISA kit offers a precise and reliable method to quantify Cathepsin D concentrations in biological samples, enabling researchers to explore its involvement in disease mechanisms and potential therapeutic strategies. Manufactured with stringent quality control standards, the Rat CTSD ELISA Kit ensures exceptional sensitivity, specificity, and reproducibility of results. Its user-friendly protocol, robust performance, and precise measurements make it a valuable asset for research studies focusing on the biological and pathological implications of Cathepsin D in various cellular processes and diseases.

Product Name:Rat CTSD (Cathepsin D) ELISA Kit
Product Code:AEES00346
Assay Type:Sandwich
Format:96T
Assay Time:3.5h
Reactivity:Rat
Detection Range:15.63-1000 pg/mL
Sensitivity:9.38 pg/mL
Sample Type & Sample Volume:Serum, plasma and other biological fluids, 100μL
Specificity:This kit recognizes Rat CTSD in samples. No significant cross-reactivity or interference between Rat CTSD and analogues was observed.
Reproducibility:Both intra-CV and inter-CV are < 10%.
Application:This ELISA kit applies to the in vitro quantitative determination of Rat CTSD concentrations in serum, plasma and other biological fluids.

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat CTSD. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CTSD and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CTSD, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat CTSD. You can calculate the concentration of Rat CTSD in the samples by comparing the OD of the samples to the standard curve.

Kit Components:

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

ItemSpecificationsStorage
Micro ELISA Plate(Dismountable)8 wells x 12 strips-20℃, 6 months
Reference Standard2 vials
Concentrated Biotinylated Detection Ab (100×)1 vial, 120 μL
Concentrated HRP Conjugate (100×)1 vial, 120 μL-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent1 vial, 20 mL2-8°C, 6 months
Biotinylated Detection Ab Diluent1 vial, 14 mL
HRP Conjugate Diluent1 vial, 14 mL
Concentrated Wash Buffer (25×)1 vial, 30 mL
Substrate Reagent1 vial, 10 mL2-8℃(Protect from light)
Stop Solution1 vial, 10 mL2-8℃
Plate Sealer5 pieces
Manual1 copy
Certificate of Analysis1 copy
1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
3. Aspirate and wash the plate for 3 times
4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
6. Add 50μL Stop Solution
7. Read the plate at 450nm immediately. Calculation of the results

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