The Rat CTSD (Cathepsin D) CLIA Kit is specifically designed for the quantitative measurement of Cathepsin D levels in rat samples, including serum, plasma, and cell culture supernatants. This kit is characterized by its high sensitivity and specificity, ensuring accurate and reproducible results for various research applications.Cathepsin D is a lysosomal protease that plays a critical role in the degradation of intracellular proteins and the regulation of cell growth and death. Dysregulation of Cathepsin D has been implicated in various diseases, including cancer, Alzheimer's disease, and cardiovascular disorders, highlighting its importance as a potential biomarker for disease progression and therapeutic development.
Overall, the Rat CTSD CLIA Kit provides researchers with a valuable tool for studying the role of Cathepsin D in disease pathology and exploring potential treatment strategies. Its reliability and precision make it an essential component of any research project involving Cathepsin D analysis in rat samples.
Product Name:
Rat CTSD (Cathepsin D) CLIA Kit
SKU:
RTES00101
Target:
Rat CTSD (Cathepsin D)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat CTSD. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CTSD and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CTSD, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat CTSD. You can calculate the concentration of Rat CTSD in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-103
93-108
92-104
Average (%)
94
99
99
1:4
Range (%)
97-112
86-97
104-121
Average (%)
105
91
110
1:8
Range (%)
90-102
102-114
89-103
Average (%)
96
107
95
1:16
Range (%)
98-113
103-120
87-99
Average (%)
104
109
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-102
95
EDTA plasma (n=5)
85-100
92
Cell culture media (n=5)
84-98
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
23.43
58.78
171.87
24.29
56.58
185.13
Standard deviation
2.54
5.81
16.29
3.04
5.04
15.88
C V (%)
10.84
9.88
9.48
12.52
8.91
8.58
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat CTSD concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat CTSD in samples. No significant cross-reactivity or interference between Rat CTSD and analogues was observed.
