Rat cTnT/TNNT2 (Troponin T Type 2, Cardiac) ELISA Kit
The Rat cTnT/TNNT2 (Troponin T Type 2, Cardiac) ELISA Kit is meticulously designed for the precise quantitative detection of Troponin T Type 2 levels in various biological samples.
Troponin T Type 2, a specific marker of cardiac injury, is crucial in the diagnosis and assessment of cardiac conditions, particularly myocardial infarction and myocardial damage. Elevated levels of cTnT are indicative of cardiac muscle damage, making it an essential biomarker in cardiology research and clinical practice. Accurate measurement of cTnT/TNNT2 is paramount for early diagnosis of cardiac pathologies, monitoring treatment efficacy, and predicting patient outcomes. This ELISA kit offers exceptional sensitivity and specificity, ensuring reliable and reproducible results. Manufactured under strict quality control measures, the kit guarantees robust performance and user-friendly operation, making it an ideal choice for cardiovascular research and diagnostic applications.
Product Name:
Rat cTnT/TNNT2 (Troponin T Type 2, Cardiac) ELISA Kit
SKU:
AEES00340
Target:
Rat cTnT/TNNT2 (Troponin T Type 2, Cardiac)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat cTnT/TNNT2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat cTnT/TNNT2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat cTnT/TNNT2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat cTnT/TNNT2. You can calculate the concentration of Rat cTnT/TNNT2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-104
97-114
85-99
Average (%)
96
105
90
1:4
Range (%)
93-106
86-101
87-98
Average (%)
99
93
93
1:8
Range (%)
91-103
83-94
87-98
Average (%)
97
89
92
1:16
Range (%)
86-99
80-90
82-93
Average (%)
92
85
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-97
91
EDTA plasma (n=5)
95-108
100
Cell culture media (n=5)
89-102
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
53.47
114.59
416.54
57.54
117.04
389.47
Standard deviation
2.78
6.38
22.16
3.02
6.31
17.92
C V (%)
5.2
5.57
5.32
5.25
5.39
4.6
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat cTnT/TNNT2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat cTnT/TNNT2 in samples. No significant cross-reactivity or interference between Rat cTnT/TNNT2 and analogues was observed.
