Rat CTGF (Connective Tissue Growth Factor) ELISA Kit
The Rat CTGF (Connective Tissue Growth Factor) ELISA Kit is a cutting-edge assay tool specifically designed for the precise quantitative detection of CTGF levels in various rat biological samples. CTGF, a key modulator of cellular activities related to cell growth, differentiation, and tissue repair, plays a fundamental role in mediating various signaling cascades that influence connective tissue functions and remodeling processes.
With this ELISA kit, researchers can accurately quantify CTGF levels, gaining valuable insights into the molecular mechanisms governing tissue growth, regeneration, and fibrotic disorders. CTGF is an essential regulatory protein that orchestrates intricate cellular responses critical for tissue homeostasis and repair. By quantifying CTGF levels with high accuracy, researchers can unravel the intricate pathways associated with connective tissue growth, fibrosis, and other cellular processes orchestrated by this growth factor. This detailed analysis facilitates a deeper understanding of connective tissue biology, pathophysiology, and potential therapeutic interventions. The Rat CTGF (Connective Tissue Growth Factor) ELISA Kit from Assay Genie is characterized by exceptional sensitivity and specificity, ensuring dependable and reproducible results. Manufactured under stringent quality control standards, this ELISA kit delivers reliable performance and user-friendly protocols, making it an ideal choice for researchers investigating the molecular mechanisms governing tissue growth and fibrosis.
Product Name:
Rat CTGF (Connective Tissue Growth Factor) ELISA Kit
SKU:
AEES00368
Target:
Rat CTGF (Connective Tissue Growth Factor)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat CTGF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CTGF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CTGF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat CTGF. You can calculate the concentration of Rat CTGF in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-112
84-95
94-110
Average (%)
105
90
101
1:4
Range (%)
91-103
83-98
86-98
Average (%)
98
89
92
1:8
Range (%)
89-103
82-96
84-98
Average (%)
95
89
90
1:16
Range (%)
91-106
83-97
84-97
Average (%)
97
90
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-103
98
EDTA plasma (n=5)
88-102
95
Cell culture media (n=5)
95-108
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
98.72
319.71
739.01
107.45
320.89
786.74
Standard deviation
6.54
17.33
29.41
6.59
14.73
28.72
C V (%)
6.62
5.42
3.98
6.13
4.59
3.65
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat CTGF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat CTGF in samples. No significant cross-reactivity or interference between Rat CTGF and analogues was observed.
