The Rat CT (Calcitonin) CLIA Kit is specifically designed for the precise measurement of calcitonin levels in rat serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results for a variety of research purposes.Calcitonin is a hormone essential for regulating calcium levels in the body and plays a critical role in bone metabolism. It is frequently used as a biomarker for various bone-related conditions, including osteoporosis and Paget's disease, as well as for monitoring thyroid health.
With its reliable performance and broad applicability, the Rat CT (Calcitonin) CLIA Kit is a valuable tool for researchers studying bone health, endocrine disorders, and other related fields. Get precise measurements and insightful data with this advanced kit from Assay Genie.
Product Name:
Rat CT (Calcitonin) CLIA Kit
SKU:
RTES00029
Target:
Rat CT (Calcitonin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat CT. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CT and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CT, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat CT. You can calculate the concentration of Rat CT in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-104
94-109
91-105
Average (%)
95
102
97
1:4
Range (%)
89-103
88-102
91-104
Average (%)
95
95
98
1:8
Range (%)
97-109
88-101
92-107
Average (%)
104
95
99
1:16
Range (%)
102-118
95-112
93-107
Average (%)
109
102
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-99
92
EDTA plasma (n=5)
93-106
100
Cell culture media (n=5)
85-99
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
26.74
72.86
205.76
24.15
71.93
217.96
Standard deviation
2.58
7.8
19.51
2.57
7.92
23.91
C V (%)
9.65
10.71
9.48
10.64
11.01
10.97
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat CT concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat CT in samples. No significant cross-reactivity or interference between Rat CT and analogues was observed.
