The Rat CRP (C-Reactive Protein) ELISA Kit is a specialized assay meticulously crafted for the precise quantitative measurement of C-Reactive Protein levels in various biological samples. C-Reactive Protein is a crucial biomarker of inflammation and is synthesized by the liver in response to systemic inflammation, infection, or tissue injury. Elevated levels of CRP have been associated with various inflammatory conditions, cardiovascular diseases, and infections, making it an indispensable marker for assessing acute-phase responses and predicting disease prognosis.
Accurate quantification of CRP is essential for understanding the inflammatory status of an individual and its significance in various pathophysiological processes. The Rat CRP ELISA Kit offered by Assay Genie provides exceptional sensitivity and specificity, ensuring accurate and reproducible results. Manufactured under stringent quality control measures, this kit delivers robust performance and user-friendly protocols, making it an excellent choice for cutting-edge research and diagnostic applications.
Product Name:
Rat CRP (C-Reactive Protein) ELISA Kit
SKU:
AEES00436
Target:
Rat CRP (C-Reactive Protein)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat CRP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CRP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CRP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat CRP. You can calculate the concentration of Rat CRP in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-110
100-113
96-108
Average (%)
102
106
102
1:4
Range (%)
100-115
86-98
92-106
Average (%)
108
93
99
1:8
Range (%)
96-110
87-100
92-108
Average (%)
101
94
99
1:16
Range (%)
93-108
83-97
95-112
Average (%)
101
90
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-103
94
EDTA plasma (n=5)
84-96
91
Cell culture media (n=5)
86-99
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.5
1.4
4.8
0.5
1.4
4.6
Standard deviation
0.03
0.06
0.24
0.03
0.08
0.22
C V (%)
6
4.29
5.0
6.0
5.71
4.78
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat CRP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat CRP in samples. No significant cross-reactivity or interference between Rat CRP and analogues was observed.
