Rat CREB1 (Cyclic AMP Response Element Binding Protein 1) ELISA Kit
The Rat CREB1 (Cyclic AMP Response Element Binding Protein 1) ELISA Kit is specifically designed for the quantitative measurement of CREB1 levels in diverse biological samples derived from rats. CREB1, a crucial transcription factor, plays a pivotal role in regulating gene expression in response to various stimuli and is implicated in a wide range of cellular processes, including cell growth, survival, and differentiation, as well as synaptic plasticity and memory consolidation.
By quantifying CREB1 levels, researchers gain insights into how this protein influences cellular signaling pathways and impacts biological functions. CREB1 is known for its involvement in numerous physiological and pathological conditions, such as neuronal plasticity, learning and memory, metabolism, circadian rhythms, and psychiatric disorders. Its intricate functions make it a key player in cellular responses to both external and internal cues, highlighting its significance in maintaining homeostasis and coordinating cellular processes. By utilizing the Rat CREB1 ELISA Kit, researchers can accurately assess CREB1 levels, enabling detailed investigations into its roles in various cellular activities and disease mechanisms. This kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results. Manufactured under strict quality control measures, the ELISA kit demonstrates robust performance and ease of use, making it an ideal tool for research studies focusing on CREB1 biology and its implications in health and disease.
Product Name:
Rat CREB1 (Cyclic AMP Response Element Binding Protein 1) ELISA Kit
SKU:
AEES00373
Target:
Rat CREB1 (Cyclic AMP Response Element Binding Protein 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 pmol/mL
Detection range:
0.31-20 pmol/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat CREB1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CREB1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CREB1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat CREB1. You can calculate the concentration of Rat CREB1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-100
89-101
90-104
Average (%)
92
94
95
1:4
Range (%)
86-100
88-100
84-97
Average (%)
92
93
91
1:8
Range (%)
88-98
83-96
85-96
Average (%)
93
89
91
1:16
Range (%)
87-101
85-98
83-95
Average (%)
94
92
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-102
97
EDTA plasma (n=5)
92-106
99
Cell culture media (n=5)
97-111
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.08
1.96
6.81
1.03
1.82
7.45
Standard deviation
0.06
0.08
0.3
0.07
0.1
0.38
C V (%)
5.56
4.08
4.41
6.8
5.49
5.1
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat CREB1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat CREB1 in samples. No significant cross-reactivity or interference between Rat CREB1 and analogues was observed.
