Rat CREB (Cyclic AMP Response Element Binding Protein) CLIA Kit
The Rat CREB (Cyclic AMP Response Element Binding Protein) CLIA Kit is a powerful tool for the quantitative measurement of CREB levels in rat samples. This kit offers high sensitivity and specificity, providing researchers with accurate and reliable results for a variety of experimental applications.CREB is a key transcription factor that plays a critical role in the regulation of gene expression in response to intracellular signaling pathways, particularly those involving cyclic AMP. It is involved in numerous cellular processes, including cell growth, differentiation, and survival, making it a valuable target for studying various physiological and pathological conditions.
With the Rat CREB CLIA Kit, researchers can explore the role of CREB in signal transduction pathways, gene regulation, and disease states in rat models. This kit is suitable for use with serum, plasma, and tissue samples, offering flexibility for different experimental designs and sample types. Trust in the accuracy and precision of the Rat CREB CLIA Kit for your research needs.
Product Name:
Rat CREB (Cyclic AMP Response Element Binding Protein) CLIA Kit
SKU:
RTES00161
Target:
Rat CREB (Cyclic AMP Response Element Binding Protein)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 fmol/mL
Detection range:
62.50-4000 fmol/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat CREB1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CREB1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CREB1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat CREB1. You can calculate the concentration of Rat CREB1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-113
87-100
86-98
Average (%)
104
94
91
1:4
Range (%)
86-99
102-117
93-110
Average (%)
91
109
101
1:8
Range (%)
103-118
91-104
95-109
Average (%)
109
98
102
1:16
Range (%)
91-104
99-115
102-116
Average (%)
96
105
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-101
96
EDTA plasma (n=5)
99-114
106
Cell culture media (n=5)
97-108
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
187.57
625.57
1678.4
202.45
609.19
1761.83
Standard deviation
24.07
55.86
173.88
24.84
51.9
179.53
C V (%)
12.83
8.93
10.36
12.27
8.52
10.19
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat CREB1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat CREB1 in samples. No significant cross-reactivity or interference between Rat CREB1 and analogues was observed.
