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Rat Creatine Kinase, Muscle (CKM) ELISA Kit

SKU:
RTDL00215
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P00564
Sensitivity:
0.63ng/mL
Range:
1.56-100ng/mL
ELISA Type:
Sandwich
Synonyms:
CK-MM,CKMM,CK3,CK-M,MM-CK,M-CK,Creatine kinase M-type, N-terminally processed
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Rat Creatine Kinase, Muscle (CKM) ELISA Kit

The Rat Creatine Kinase Muscle (CKM) ELISA Kit is specifically designed for the precise measurement of CKM levels in rat serum, plasma, and tissue lysates. With its exceptional sensitivity and specificity, this kit provides researchers with reliable and consistent results, making it an invaluable tool for a variety of scientific studies.Creatine Kinase Muscle (CKM) is an essential enzyme mainly found in muscle tissues, playing a key role in energy metabolism and muscle contraction.

Abnormal levels of CKM have been associated with various muscle-related disorders, making it a valuable biomarker for studying muscle function and health.Whether investigating muscle damage, muscle diseases, or evaluating the impact of therapeutic interventions, the Rat CKM ELISA Kit offers a reliable and accurate method for measuring CKM levels in rat samples. Trust in this kit to deliver trustworthy data for your research needs.

Product Name:Rat Creatine Kinase, Muscle (CKM) ELISA Kit
Product Code:RTDL00215
Size:96 Assays
Target:CKM
Synonyms:CK-MM, CKMM, CK3, CK-M, MM-CK, M-CK, Creatine kinase M-type, N-terminally processed
Detection Method:Sandwich
Range:1.56-100ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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