The Rat CP (Ceruloplasmin) ELISA Kit is a specialized assay designed for the precise and quantitative measurement of ceruloplasmin levels in rat biological samples. Ceruloplasmin is a multi-functional ferroxidase enzyme involved in various physiological processes, including copper metabolism, iron transport, and antioxidant defense. With pivotal roles in iron homeostasis and oxidative stress regulation, ceruloplasmin serves as an essential participant in maintaining cellular health and systemic balance. Accurate determination of ceruloplasmin levels provided by this ELISA kit is crucial for studying its involvement in iron metabolism disorders, neurological diseases, inflammation, and cardiovascular conditions.
Changes in ceruloplasmin expression or activity can have significant implications for overall health and may serve as biomarkers for various pathologies. The Rat CP ELISA Kit from Assay Genie offers exceptional sensitivity and specificity, ensuring precise and reproducible results. Manufactured under stringent quality control measures, this kit delivers reliable performance, making it an ideal choice for research applications. Its user-friendly format and robust analytical capabilities make it well-suited for investigating the intricate functions of ceruloplasmin in physiological and pathological contexts.
Product Name:
Rat CP (Ceruloplasmin) ELISA Kit
SKU:
AEES00348
Target:
Rat CP (Ceruloplasmin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
3.75 ng/mL
Detection range:
6.25-400 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat CP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat CP. You can calculate the concentration of Rat CP in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-96
95-110
92-107
Average (%)
91
103
100
1:4
Range (%)
90-102
83-95
89-103
Average (%)
96
89
94
1:8
Range (%)
90-104
81-94
82-93
Average (%)
95
88
88
1:16
Range (%)
88-100
81-96
85-97
Average (%)
93
87
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-111
102
EDTA plasma (n=5)
85-95
90
Cell culture media (n=5)
95-109
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
21.14
36.95
133.84
21.92
39.64
122.12
Standard deviation
1.36
1.95
5.06
1.37
1.64
5.94
C V (%)
6.43
5.28
3.78
6.25
4.14
4.86
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat CP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat CP in samples. No significant cross-reactivity or interference between Rat CP and analogues was observed.
