The Rat CP (Ceruloplasmin) CLIA Kit is a highly reliable and sensitive assay kit designed for the accurate detection of ceruloplasmin levels in rat samples. This kit allows for the precise measurement of ceruloplasmin levels in serum, plasma, and cell culture supernatants, making it suitable for a variety of research applications.Ceruloplasmin is an essential ferroxidase enzyme that plays a critical role in iron metabolism and antioxidant defense. Abnormal ceruloplasmin levels have been linked to various diseases and disorders, including Wilson's disease, Alzheimer's disease, and cardiovascular conditions.
By measuring ceruloplasmin levels accurately, researchers can gain valuable insights into the mechanisms underlying these conditions and potentially develop new therapeutic strategies.With its high sensitivity, specificity, and reproducibility, the Rat CP (Ceruloplasmin) CLIA Kit offers researchers a powerful tool for studying the role of ceruloplasmin in health and disease. Get reliable and accurate results with this advanced assay kit from Assay Genie.
Product Name:
Rat CP (Ceruloplasmin) CLIA Kit
SKU:
RTES00105
Target:
Rat CP (Ceruloplasmin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.75 ng/mL
Detection range:
1.25-80 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat CP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat CP. You can calculate the concentration of Rat CP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-114
99-114
103-115
Average (%)
106
104
109
1:4
Range (%)
93-108
87-100
90-104
Average (%)
101
93
97
1:8
Range (%)
99-111
96-106
95-111
Average (%)
105
101
103
1:16
Range (%)
86-97
94-105
95-113
Average (%)
92
99
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-112
106
EDTA plasma (n=5)
92-103
98
Cell culture media (n=5)
90-102
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
3.78
12.55
27.73
3.62
12.05
27.66
Standard deviation
0.48
1.17
3.17
0.33
1.32
2.93
C V (%)
12.7
9.32
11.43
9.12
10.95
10.59
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat CP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat CP in samples. No significant cross-reactivity or interference between Rat CP and analogues was observed.
