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Rat Corin (CRN) ELISA Kit

SKU:
RTDL00254
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q80YN4
Sensitivity:
0.065ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
ATC2,CRN,Lrp4,TMPRSS10,Atrial Natriuretic Peptide-Converting Enzyme, Serine Protease,Heart-specific serine proteinase ATC2,Pro-ANP-converting enzyme
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€749
Frequently bought together:

Description

system_update_altDatasheet

Rat Corin (CRN) ELISA Kit

The Rat Corin (CRN) ELISA Kit is a highly reliable and sensitive assay designed for the accurate detection of corin levels in rat samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional specificity, ensuring reproducible results that are ideal for various research applications.Corin is a key enzyme involved in the regulation of cardiac function and blood pressure by activating natriuretic peptides. Dysregulation of corin has been linked to various cardiovascular diseases, making it a valuable biomarker for studying these conditions and potentially developing new therapeutic interventions.

With its high sensitivity and specificity, the Rat Corin (CRN) ELISA Kit is a valuable tool for researchers studying cardiovascular diseases and looking to understand the role of corin in these conditions. Get accurate and reliable results with this kit from Assay Genie.

Product Name:Rat Corin (CRN) ELISA Kit
Product Code:RTDL00254
Size:96 Assays
Target:CRN
Synonyms:ATC2, CRN, Lrp4, TMPRSS10, Atrial Natriuretic Peptide-Converting Enzyme, Serine Protease
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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