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Rat Complement Factor D (CFD) ELISA Kit

SKU:
RTDL00203
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P32038
Sensitivity:
0.27ng/mL
Range:
0.781-50ng/mL
ELISA Type:
Sandwich
Synonyms:
CF-D,DF,PFD,ADN,Adipsin,Properdin Factor D,C3 convertase activator
Reactivity:
Rat
Sample Type:
Serum, Plasma And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Rat Complement Factor D (CFD) ELISA Kit

The Rat Complement Factor D/Cfd ELISA Kit is specifically designed for the precise measurement of complement factor D levels in rat serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and accuracy, guaranteeing consistent and trustworthy results for various research purposes.Complement factor D is a key protein in the complement system, playing a crucial role in the activation of the alternative pathway. Dysregulation of complement factor D has been implicated in various inflammatory and immune-related diseases, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.

Overall, the Rat Complement Factor D/Cfd ELISA Kit is an essential tool for researchers looking to accurately quantify complement factor D levels in rat samples, providing valuable insights into the role of this protein in health and disease.

Product Name:Rat Complement Factor D (CFD) ELISA Kit
Product Code:RTDL00203
Size:96 Assays
Target:CFD
Synonyms:CF-D, DF, PFD, ADN, Adipsin, Properdin Factor D, C3 convertase activator
Detection Method:Sandwich
Range:0.78-50ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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