Rat COMP (Cartilage Oligomeric Matrix Protein) ELISA Kit
The Rat COMP (Cartilage Oligomeric Matrix Protein) ELISA Kit is specifically designed for the quantitative detection of COMP levels in rat biological samples.
COMP is a key structural component of the extracellular matrix in cartilage and other tissues, playing a critical role in maintaining tissue integrity and function. Quantifying COMP levels is crucial for investigating cartilage health, joint diseases, and musculoskeletal disorders. Our ELISA kit provides exceptional sensitivity and specificity, ensuring accurate and reproducible results for your research needs. Manufactained under strict quality control measures, this kit offers robust performance and user-friendly protocols, making it a valuable tool for studying cartilage biology, disease mechanisms, and therapeutic interventions.
Product Name:
Rat COMP (Cartilage Oligomeric Matrix Protein) ELISA Kit
SKU:
AEES00343
Target:
Rat COMP (Cartilage Oligomeric Matrix Protein)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat COMP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat COMP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat COMP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat COMP. You can calculate the concentration of Rat COMP in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-106
99-112
99-111
Average (%)
97
104
104
1:4
Range (%)
92-104
87-98
85-99
Average (%)
97
92
91
1:8
Range (%)
88-101
88-98
83-96
Average (%)
93
93
90
1:16
Range (%)
91-102
88-98
85-98
Average (%)
97
93
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-107
101
EDTA plasma (n=5)
93-107
99
Cell culture media (n=5)
88-99
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
5.25
10.89
43.87
5.28
10.04
39.84
Standard deviation
0.36
0.52
1.41
0.27
0.47
1.33
C V (%)
6.86
4.78
3.21
5.11
4.68
3.34
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat COMP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat COMP in samples. No significant cross-reactivity or interference between Rat COMP and analogues was observed.
