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Rat Colony Stimulating Factor 1, Macrophage (MCSF) ELISA Kit

SKU:
RTDL00658
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q8JZQ0
Sensitivity:
5.9pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
CSF1,M-CSF,Lanimostim,Processed macrophage colony-stimulating factor 1
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Rat Colony Stimulating Factor 1, Macrophage (MCSF) ELISA Kit

The Rat Colony Stimulating Factor-1 (Rat CSF-1) Macrophage ELISA Kit is specifically designed for the accurate and sensitive detection of Rat CSF-1 levels in rat serum, plasma, and cell culture supernatants. This kit offers high precision and reliability, ensuring consistent and reproducible results for a variety of research applications.Rat CSF-1 is a critical factor in the differentiation, proliferation, and survival of macrophages, playing a key role in the immune response and inflammation.

This protein is essential for maintaining homeostasis in the immune system and is involved in various physiological and pathological processes.By accurately measuring Rat CSF-1 levels, researchers can gain valuable insights into the mechanisms underlying immune responses, inflammatory diseases, and cancer progression. This ELISA kit provides a powerful tool for studying the role of Rat CSF-1 in various biological processes and developing potential therapeutic interventions.

Product Name:Rat Colony Stimulating Factor 1, Macrophage (MCSF) ELISA Kit
Product Code:RTDL00658
Size:96 Assays
Target:MCSF
Synonyms:CSF1, M-CSF, Lanimostim, Processed macrophage colony-stimulating factor 1
Detection Method:Sandwich
Range:15.625-1000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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