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Rat Collagen Type II Alpha 1 (COL2a1) ELISA Kit

SKU:
RTDL00230
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P05539
Sensitivity:
18.25pg/mL
Range:
46.875-3000pg/mL
ELISA Type:
Sandwich
Synonyms:
COL2-A1,COL2A1,SEDC,Chondrocalcin,Collagen Alpha-1(II)Chain,Primary Osteoarthritis,Spondyloepiphyseal Dysplasia,Congenital
Reactivity:
Rat
Sample Type:
Serum, Plasma And Other Biological Fluids
$899
Frequently bought together:

Description

system_update_altDatasheet

Rat Collagen Type II Alpha 1 (COL2a1) ELISA Kit

The Rat Collagen Type II Alpha-1 (COL2A1) ELISA Kit offered by AssayGenie is a valuable tool for detecting levels of COL2A1 in rat serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.COL2A1 is a critical component of type II collagen, an essential protein in cartilage and connective tissue. Abnormalities in COL2A1 expression can lead to various musculoskeletal disorders, including arthritis and osteoarthritis.

By accurately measuring COL2A1 levels, researchers can gain valuable insights into the pathophysiology of these conditions and aid in the development of potential treatments.Whether investigating cartilage regeneration, joint health, or musculoskeletal diseases, the Rat Collagen Type II Alpha-1 (COL2A1) ELISA Kit from AssayGenie is a valuable tool for researchers seeking to advance their understanding of COL2A1 biology.

Product Name:Rat Collagen Type II Alpha 1 (COL2a1) ELISA Kit
Product Code:RTDL00230
Size:96 Assays
Target:COL2a1
Synonyms:COL2-A1, COL2A1, SEDC, Chondrocalcin, Collagen Alpha-1(II)Chain, Primary Osteoarthritis,Spondyloepiphyseal Dysplasia,Congenital
Detection Method:Sandwich
Range:46.875-3000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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