Rat COL9 alpha1 (Collagen Type IX Alpha 1) CLIA Kit
The Rat COL9 Alpha1 (Collagen Type IX Alpha 1) CLIA Kit is a reliable and accurate tool for the detection of COL9 Alpha1 levels in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for various research applications.COL9 Alpha1 is a key component of collagen Type IX, contributing to the structure and function of cartilage in the body.
Abnormalities in COL9 Alpha1 have been associated with osteoarthritis and other joint-related disorders, highlighting its importance as a biomarker in the study of these conditions.With the Rat COL9 Alpha1 CLIA Kit, researchers can confidently analyze COL9 Alpha1 levels in rat samples, furthering our understanding of cartilage biology and potentially leading to the development of novel therapies for joint diseases.
Product Name:
Rat COL9 alpha1 (Collagen Type IX Alpha 1) CLIA Kit
SKU:
RTES00142
Target:
Rat COL9 alpha1 (Collagen Type IX Alpha 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat COL9A 1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat COL9A 1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat COL9A 1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat COL9A 1. You can calculate the concentration of Rat COL9A 1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-110
100-113
100-111
Average (%)
103
106
105
1:4
Range (%)
97-109
99-115
91-105
Average (%)
102
107
96
1:8
Range (%)
91-104
90-103
98-115
Average (%)
97
97
106
1:16
Range (%)
96-110
98-112
86-97
Average (%)
104
104
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-105
97
EDTA plasma (n=5)
85-98
92
Cell culture media (n=5)
84-98
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.98
3.05
9.39
0.91
2.94
8.5
Standard deviation
0.08
0.33
0.7
0.11
0.33
0.88
C V (%)
8.16
10.82
7.45
12.09
11.22
10.35
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat COL9A 1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat COL9A 1 in samples. No significant cross-reactivity or interference between Rat COL9A 1 and analogues was observed.
