The Rat Collagen Type IV (COL4) ELISA Kit is a specialized assay designed for the quantitative measurement of Collagen Type IV levels in rat biological samples.
Collagen Type IV is a critical structural protein that forms a major component of the basement membrane, contributing to tissue integrity, filtration barrier function, and cell adhesion. This ELISA kit provides researchers with a powerful tool to investigate the regulation of Collagen Type IV in various physiological and pathological conditions. Accurate quantification of Collagen Type IV levels is essential for studying basement membrane biology, extracellular matrix remodeling, and tissue-specific functions. The Rat COL4 ELISA Kit enables researchers to explore the role of Collagen Type IV in tissue development, repair processes, and disease pathogenesis, offering valuable insights into the mechanisms underlying basement membrane-related disorders and tissue homeostasis. By leveraging the exceptional sensitivity and specificity of the Rat COL4 ELISA Kit, researchers can obtain reliable and reproducible results, ensuring high-quality data for comprehensive investigations into Collagen Type IV dynamics and its impact on cellular interactions and tissue function. Trust in the robust performance of this kit to advance research in collagen biology, extracellular matrix regulation, and basement membrane integrity.
Product Name:
Rat COL4 (Collagen Type Ⅳ) ELISA Kit
SKU:
AEES00525
Target:
Rat COL4 (Collagen Type Ⅳ)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat COL4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat COL4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat COL4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat COL4. You can calculate the concentration of Rat COL4 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-106
89-99
92-104
Average (%)
98
95
99
1:4
Range (%)
92-104
88-100
94-102
Average (%)
97
95
98
1:8
Range (%)
91-103
85-100
89-103
Average (%)
98
93
95
1:16
Range (%)
85-93
95-107
97-105
Average (%)
90
103
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-112
104
EDTA plasma (n=5)
89-99
95
Cell culture media (n=5)
96-109
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.39
5.62
21.36
2.42
5.47
23.12
Standard deviation
0.13
0.27
0.87
0.14
0.26
1.16
C V (%)
5.44
4.8
4.07
5.79
4.75
5.02
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat COL4 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat COL4 in samples. No significant cross-reactivity or interference between Rat COL4 and analogues was observed.
