The Rat COL3 (Collagen Type III) ELISA Kit is an advanced research tool designed for the quantitative determination of Collagen Type III levels in a variety of rat biological samples.
Collagen Type III is a crucial structural protein that plays a significant role in providing structural support and elasticity to connective tissues, including skin, blood vessels, and internal organs. This ELISA kit enables accurate measurement of Collagen Type III concentrations, facilitating insights into tissue remodeling, wound healing, and fibrotic processes. Precise quantification of Collagen Type III levels is vital for understanding its contribution to tissue integrity, elasticity, and repair mechanisms. By utilizing the Rat COL3 ELISA Kit, researchers can investigate the dynamics of Collagen Type III expression, explore its involvement in tissue remodeling processes, and elucidate its significance in various physiological and pathological conditions. The Rat COL3 ELISA Kit from Assay Genie offers exceptional sensitivity and specificity, ensuring accurate and reproducible results. Manufactured under stringent quality control measures, this kit delivers robust and reliable performance, making it an ideal choice for research projects focused on unraveling the biological roles of Collagen Type III in health and disease.
Product Name:
Rat COL3 (Collagen Type Ⅲ) ELISA Kit
SKU:
AEES00360
Target:
Rat COL3 (Collagen Type Ⅲ)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat COL3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat COL3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat COL3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat COL3. You can calculate the concentration of Rat COL3 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-105
95-106
85-100
Average (%)
96
100
91
1:4
Range (%)
87-100
80-89
86-100
Average (%)
92
85
92
1:8
Range (%)
92-104
83-97
87-97
Average (%)
99
89
92
1:16
Range (%)
91-102
86-97
88-100
Average (%)
96
91
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-102
95
EDTA plasma (n=5)
94-106
100
Cell culture media (n=5)
94-109
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
216.81
334.18
1952.24
213.45
338.11
1998.02
Standard deviation
12.49
17.61
92.93
11.78
20.22
64.74
C V (%)
5.76
5.27
4.76
5.52
5.98
3.24
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rat COL3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat COL3 in samples. No significant cross-reactivity or interference between Rat COL3 and analogues was observed.
