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Rat Coagulation Factor XI (F11) ELISA Kit

SKU:
RTDL00345
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q6TUF8
Sensitivity:
0.067ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
FXI,PTA,Fletcher Factor,Prekallikrein,Plasma Thromboplastin Antecedent
Reactivity:
Rat
Sample Type:
Plasma And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Rat Coagulation Factor XI (F11) ELISA Kit

The Rat Coagulation Factor XI (F11) ELISA Kit is specifically designed for the precise measurement of rat F11 levels in serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results for various research purposes.Coagulation Factor XI is a key protein involved in the coagulation cascade, playing a crucial role in blood clot formation. Dysregulation of F11 levels has been linked to various blood disorders, making it a valuable biomarker for studying coagulation disorders and developing therapeutic interventions.

With this ELISA kit, researchers can effectively study the role of F11 in coagulation processes and its implications in diseases such as hemophilia and thrombosis. The reliable performance of this kit makes it an essential tool for advancing research in the field of coagulation and hemostasis.

Product Name:Rat Coagulation Factor XI (F11) ELISA Kit
Product Code:RTDL00345
Size:96 Assays
Target:F11
Synonyms:FXI, PTA, Fletcher Factor, Prekallikrein, Plasma Thromboplastin Antecedent
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10296
Heparin plasma (n=5)89-10295
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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