The Rat CNTF (Ciliary Neurotrophic Factor) CLIA Kit is specifically designed for the accurate and reliable detection of CNTF levels in rat serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.CNTF is a key neurotrophic factor that plays a critical role in promoting the survival and differentiation of various neuronal populations. It is involved in the regulation of neural development, synaptic plasticity, and neuroprotection.
Dysregulation of CNTF has been implicated in various neurodegenerative disorders, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.Overall, the Rat CNTF CLIA Kit provides researchers with a powerful tool for studying the role of CNTF in neurobiology and disease pathology, ultimately leading to a better understanding of neuroprotection and potential treatment strategies.
Product Name:
Rat CNTF (Ciliary Neurotrophic Factor) CLIA Kit
SKU:
RTES00120
Target:
Rat CNTF (Ciliary Neurotrophic Factor)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat CNTF. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CNTF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CNTF, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat CNTF. You can calculate the concentration of Rat CNTF in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-102
91-105
92-105
Average (%)
93
98
97
1:4
Range (%)
101-114
94-110
90-105
Average (%)
107
101
97
1:8
Range (%)
90-103
99-116
92-105
Average (%)
96
107
100
1:16
Range (%)
99-111
90-103
95-108
Average (%)
105
96
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-109
100
EDTA plasma (n=5)
95-106
101
Cell culture media (n=5)
96-109
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
37.35
92.2
304.5
38.82
89.7
308.06
Standard deviation
3.2
8.0
27.34
3.64
7.94
28.31
C V (%)
8.57
8.68
8.98
9.38
8.85
9.19
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat CNTF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat CNTF in samples. No significant cross-reactivity or interference between Rat CNTF and analogues was observed.
