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Rat CNP (C-Type Natriuretic Peptide) ELISA Kit

SKU:
AEES00372
Product Type:
ELISA Kit
ELISA Type:
Competitive
Size:
96 Assays
Reactivity:
Rat
Sensitivity:
9.38 pg/mL
Range:
15.63-1000 pg/mL
Sample Type:
Serum, plasma and other biological fluids
€499
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Description

Rat CNP (C-Type Natriuretic Peptide) ELISA Kit

The Rat CNP (C-Type Natriuretic Peptide) ELISA Kit is a cutting-edge assay designed to quantitatively measure the levels of CNP in various biological samples from rats.

CNP, a member of the natriuretic peptide family, is a crucial regulator of cardiovascular function, vascular tone, and renal physiology. As a potent vasodilator and modulator of renal sodium excretion, CNP plays a vital role in maintaining blood pressure, circulatory homeostasis, and cardiovascular health. This ELISA kit enables researchers to accurately assess CNP concentrations, providing valuable insights into cardiovascular physiology, hypertension, and renal function. CNP is recognized for its cardioprotective, vasodilatory, and antifibrotic properties, making it a key player in cardiovascular health and disease. Measurement of CNP levels can offer valuable information on the pathophysiology of cardiovascular disorders, hypertension, and vascular diseases. By utilizing the Rat CNP ELISA Kit, researchers can gain a deeper understanding of CNP's biological functions, cardiovascular effects, and its implications in cardiac health and disease management. The Rat CNP (C-Type Natriuretic Peptide) ELISA Kit from Assay Genie ensures exceptional sensitivity and specificity, providing accurate and reproducible results with high precision. Manufactured under rigorous quality control standards, this kit offers robust performance and user-friendly protocols, making it an excellent choice for investigating CNP levels in cardiovascular research, hypertension studies, and biomarker discovery.

Product Name:Rat CNP (C-Type Natriuretic Peptide) ELISA Kit
Product Code:AEES00372
Assay Type:Competitive
Format:96T
Assay Time:2.0h
Reactivity:Rat
Detection Range:15.63-1000 pg/mL
Sensitivity:9.38 pg/mL
Sample Type & Sample Volume:Serum, plasma and other biological fluids, 50μL
Specificity:This kit recognizes CNP in samples. No significant cross-reactivity or interference between CNP and analogues was observed.
Reproducibility:Both intra-CV and inter-CV are < 10%.
Application:This ELISA kit applies to the in vitro quantitative determination of CNP concentrations in serum, plasma and other biological fluids.

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with CNP. During the reaction, CNP in the sample or standard competes with a fixed amount of CNP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to CNP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CNP in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Kit Components:

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

ItemSpecificationsStorage
Micro ELISA Plate(Dismountable)8 wells x 12 strips-20℃, 6 months
Reference Standard2 vials
Concentrated Biotinylated Detection Ab (100×)1 vial, 120 μL
Concentrated HRP Conjugate (100×)1 vial, 120 μL-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent1 vial, 20 mL2-8°C, 6 months
Biotinylated Detection Ab Diluent1 vial, 14 mL
HRP Conjugate Diluent1 vial, 14 mL
Concentrated Wash Buffer (25×)1 vial, 30 mL
Substrate Reagent1 vial, 10 mL2-8℃(Protect from light)
Stop Solution1 vial, 10 mL2-8℃
Plate Sealer5 pieces
Manual1 copy
Certificate of Analysis1 copy
1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C
2. Aspirate and wash the plate for 3 times
3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
5. Add 50μL Stop Solution
6. Read the plate at 450nm immediately. Calculation of the results

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