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Rat Cluster Of Differentiation 4 (CD4) ELISA Kit

SKU:
RTDL00192
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P05540
Sensitivity:
0.056ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
CD4mut,p55,T-cell surface glycoprotein CD4,T-cell surface antigen T4/Leu-3
Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Rat Cluster Of Differentiation 4 (CD4) ELISA Kit

The Rat Cluster of Differentiation 4 (CD4) ELISA Kit is a reliable and accurate tool for measuring CD4 levels in rat serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit provides precise and reproducible results for a variety of research applications.CD4 is a vital protein that plays a key role in the immune system by helping to regulate and control the body's adaptive immune response.

Monitoring CD4 levels is essential for studying immune function, infections, autoimmune diseases, and immunodeficiency disorders. This ELISA kit offers a valuable tool for researchers interested in investigating these areas and developing potential therapies.

Product Name:Rat Cluster Of Differentiation 4 (CD4) ELISA Kit
Product Code:RTDL00192
Size:96 Assays
Target:CD4
Synonyms:CD4mut, p55, T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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